Caspase-1 Activity Assay Kit (Colorimetric)

Images

 
Caspase Colorimetric Assay Kit [NBP2-54815] - (a) Active recombinant caspase-1 standard curve. (b) Caspase-1 activity in 1 ug uninduced and induced Jurkat cell lysate. Assay was performed according to the kit protocol.

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Suitable Sample Type
Cell and tissue lysates

Order Details

Caspase-1 Activity Assay Kit (Colorimetric) Summary

Description
Simple and convenient colorimetric assay to measure Caspase-1 activity within 1-2 hrs. Detection method: Absorbance at 400 or 405 nm. Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Kit Type
Activity Assay Kit (Colorimetric)
Gene
CASP1

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. 2X Reaction Buffer
  2. Cell Lysis Buffer
  3. DTT (1 M)
  4. Dilution Buffer
  5. YVAD-pNA (4 mM)

Alternate Names for Caspase-1 Activity Assay Kit (Colorimetric)

  • CASP1
  • CASP-1
  • caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta
  • caspase 1, apoptosis-related cysteine protease (interleukin 1, beta
  • Caspase1
  • Caspase-1
  • EC 3.4.22
  • EC 3.4.22.36
  • ICE
  • ICEP45
  • IL-1 beta-converting enzyme
  • IL1BC CASP1 nirs variant 1
  • IL-1BC
  • IL1BCE
  • IL1B-convertase
  • interleukin 1-B converting enzyme
  • interleukin 1-beta convertase
  • Interleukin-1 beta convertase
  • Interleukin-1 beta-converting enzyme
  • p45

Background

Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Blogs on Caspase-1.

The inflammasome: an inflammation-initiating machine, Novus Biologicals
By Stephanie Melchor The inflammasome is a large, multimeric protein complex found primarily in innate immune cells, which are white blood cells that can attack a wide range of pathogenic threats. Three main elements...  Read full blog post.

NLRP3/NALP3 - Sensing and responding to pathogen infection
The inflammasome is a multi protein complex that is an important component of the innate immune response. The inflammasome is able to sense and respond to pathogen infections by recognizing pathogen-associated molecular patterns and mediating the ...  Read full blog post.

Caspase 10 - an initiator caspase in the extrinsic death receptor pathway
Apoptosis, also called programmed cell death, is an essential process in development and disease. The signaling networks that carry out apoptosis is consists of a series of endoproteases called caspases which are synthesized as inactive zymogens....  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 1 - activating innate immune responses following infection or injury
Caspase-1 is an enzyme involved in the conversion of interleukin-1 into its active secreted form. Interleukin-1 mediates inflammatory responses during infection and disease. Caspase-1 is recruited to and activated by the inflammasome complex (1). Un...  Read full blog post.

Caspase-4 - a human protease with roles in inflammation and immunity
Caspases are a family of cysteine-aspartic acid proteases that cleave caspase proenzymes as well as other protein substrates. Caspases are well known for their role in apoptosis, but they also play a significant role in other cellular processes inc...  Read full blog post.

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Bioinformatics

Gene Symbol CASP1