Caspase-1 Antibody

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Western Blot: Caspase-1 Antibody [NBP1-45433] - Analysis of Caspase-1 expression in Rat liver tissue lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Caspase-1 Antibody Summary

Immunogen
Synthetic peptide surrounding amino acid 132 of human caspase-1 (NP_150636).
Specificity
The immunoaffinity purified antibody recognizes 45 kDa procaspase-1 and the 20 kDa cleaved product.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-4 ug/ml
  • Immunocytochemistry/Immunofluorescence 10-20 ug/ml
  • Immunoprecipitation 4-8 ug/ml
Application Notes
Western blot analysis (0.5-4 ug/ml), immunoprecipitation (4-8 ug/ml) and immunofluorescence (10-20 ug/ml). The immunoaffinity purified antibody recognizes 45 kDa procaspase-1 and the 20 kDa cleaved product.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-45433 in the following applications:

Publications
Read Publications using
NBP1-45433 in the following applications:

  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (PBS), pH 7.2, 30% glycerol, 0.5% BSA, 0.5 mM EDTA.
Preservative
0.03% Proclin
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Caspase-1 Antibody

  • CASP1
  • CASP-1
  • caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta
  • caspase 1, apoptosis-related cysteine protease (interleukin 1, beta
  • Caspase1
  • Caspase-1
  • EC 3.4.22
  • EC 3.4.22.36
  • ICE
  • ICEP45
  • IL-1 beta-converting enzyme
  • IL1BC CASP1 nirs variant 1
  • IL-1BC
  • IL1BCE
  • IL1B-convertase
  • interleukin 1-B converting enzyme
  • interleukin 1-beta convertase
  • Interleukin-1 beta convertase
  • Interleukin-1 beta-converting enzyme
  • p45

Background

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase 1 is similar to the cell death gene CED3 of C. elegans and regulates multiple proinflammatory cytokines, including Interleukin 1b and interferon-gamma-inducing factor. Caspase 1 plays a role down stream of Caspase 8 which is involved in Fas-mediated apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-1 Antibody (NBP1-45433)(4)

Review for Caspase-1 Antibody (NBP1-45433) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-45433:
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WB
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Images Ratings Applications Species Date Details
Western Blot Caspase-1 NBP1-45433
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reviewed by:
Chunhui Sun
WB Human 07/21/2015
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Summary

ApplicationWestern Blot
Sample TestedCell lysate
SpeciesHuman

Blocking

Blocking Details5% milk, 1 hour, room temperature

Details

Detection NotesECL

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-1 Antibody (NBP1-45433) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Caspase-1 Antibody (NBP1-45433)

Discover related pathways, diseases and genes to Caspase-1 Antibody (NBP1-45433). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Caspase-1 Antibody (NBP1-45433)

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Research Areas for Caspase-1 Antibody (NBP1-45433)

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Blogs on Caspase-1.

NLRP3/NALP3 - Sensing and responding to pathogen infection
The inflammasome is a multi protein complex that is an important component of the innate immune response. The inflammasome is able to sense and respond to pathogen infections by recognizing pathogen-associated molecular patterns and mediating the ...  Read full blog post.

Caspase 10 - an initiator caspase in the extrinsic death receptor pathway
Apoptosis, also called programmed cell death, is an essential process in development and disease. The signaling networks that carry out apoptosis is consists of a series of endoproteases called caspases which are synthesized as inactive zymogens....  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 1 - activating innate immune responses following infection or injury
Caspase-1 is an enzyme involved in the conversion of interleukin-1 into its active secreted form. Interleukin-1 mediates inflammatory responses during infection and disease. Caspase-1 is recruited to and activated by the inflammasome complex (1). Un...  Read full blog post.

Caspase-4 - a human protease with roles in inflammation and immunity
Caspases are a family of cysteine-aspartic acid proteases that cleave caspase proenzymes as well as other protein substrates. Caspases are well known for their role in apoptosis, but they also play a significant role in other cellular processes inc...  Read full blog post.

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Recent Reviews

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5
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4
1
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0
2
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1
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Chunhui Sun
07/21/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CASP1
Entrez
Uniprot