Casein Kinase 1 alpha Antibody

Western Blot: Casein Kinase 1 alpha Antibody [NBP1-18880] - CKI alpha by Western Blot and Immunoprecipitation:CKI alpha Antibody [NBP1-18880]- Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CKI alpha antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. CKI alpha was also immunoprecipitated another by rabbit anti-CKI alpha antibody which recognizes an upstream epitope.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Casein Kinase 1 alpha Antibody Summary

• Description: Novus Biologicals Rabbit Casein Kinase 1 alpha Antibody (NBP1-18880) is a polyclonal antibody validated for use in WB and IP. Anti-Casein Kinase 1 alpha Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 287 and 337 of human casein kinase 1 alpha 1 (NP_001883.4).
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSNK1A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Casein Kinase 1 alpha Antibody

• Casein Kinase 1 alpha
• casein kinase 1, alpha 1
• casein kinase I isoform alpha
• CK1CKI-alpha
• CSNK1A1
• down-regulated in lung cancer
• EC 2.7.11.1
• HLCDGP1
• PRO2975

Background

Casein Kinase I alpha (CKI alpha) is a serine/threonine protein kinase involved in the wnt signaling pathway. It is part of the CK1 serine/threonine kinase family which includes CK1 alpha, CK1beta, CK1delta, CK1epsilon, and CK1gamma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Casein Kinase 1 alpha Antibody (NBP1-18880)(2)

Publications using NBP1-18880

• Goretsky T, Bradford EM, Ryu H et al. A Cytosolic Multiprotein Complex containing p85a is required for B-catenin Activation in Colitis and Colitis-associated Cancer. J. Biol. Chem. 2015-11-12 [PMID: 26565021] (IP, Human)
• Hutchinson JA, Shanware NP, Chang H et al. Regulation of ribosomal protein S6 phosphorylation by casein kinase 1 and protein phosphatase 1. J Biol Chem 2011-03-01 [PMID: 21233202]

Bioinformatics

• Gene Symbol: CSNK1A1
• Entrez:
  • 1452(Human)
  • 93687(Mouse)
• Uniprot:
  • P48729(Human)