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![Immunoprecipitation: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - CSNK1A1/CSNK1A1L was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-33146 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33146 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-33146 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-33146 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 11 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-33146_rabbit-csnk1a1-csnk1a1l-mab-psh04-58-26820241582616.jpg "Immunoprecipitation: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - CSNK1A1/CSNK1A1L was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-33146 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33146 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-33146 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-33146 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 11 seconds; ECL")
![Western Blot: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Western blot analysis of CSNK1A1/CSNK1A1L on different lysates with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/2,000 dilution. Lane 1: HCT 116 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: RAW264.7 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Rat brain tissue lysate (40 ug/Lane) Lane 11: Mouse kidney tissue lysate (40 ug/Lane) Lane 12: Rat kidney tissue lysate (40 ug/Lane) Lane 13: Mouse spleen tissue lysate (40 ug/Lane) Lane 14: Rat spleen tissue lysate (40 ug/Lane) Predicted band size: 39 kDa Observed band size: 34 kDa Exposure time: 15 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33146) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33146_rabbit-csnk1a1-csnk1a1l-mab-psh04-58-28820241430401.jpg)
![Immunohistochemistry: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33146) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33146_rabbit-csnk1a1-csnk1a1l-mab-psh04-58-29820241435251.jpg)
![Immunocytochemistry/ Immunofluorescence: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Immunocytochemistry analysis of HCT 116 cells labeling CSNK1A1/CSNK1A1L with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33146_rabbit-csnk1a1-csnk1a1l-mab-psh04-58-29202410234299.jpg)
![Flow Cytometry: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Flow cytometric analysis of HCT 116 cells labeling CSNK1A1/CSNK1A1L. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33146, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33146_rabbit-csnk1a1-csnk1a1l-mab-psh04-58-392024884665.jpg)
![Western Blot: Casein Kinase 1 alpha Antibody [NBP2-50030] - Analysis of whole rat spinal cord homogenate stained with NBP2-50030, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~38kDa and a less prominent band at ~34kDa corresponds to other Casein Kinase 1 alpha isotypes.](http://images.novusbio.com/fullsize/Casein-Kinase-1-alpha-Antibody-Western-Blot-NBP2-50030-img0002.jpg "Western Blot: Casein Kinase 1 alpha Antibody [NBP2-50030] - Analysis of whole rat spinal cord homogenate stained with NBP2-50030, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~38kDa and a less prominent band at ~34kDa corresponds to other Casein Kinase 1 alpha isotypes.")
![Immunocytochemistry/Immunofluorescence: Casein Kinase 1 alpha Antibody [NBP2-50030] - HeLa cells stained with NBP2-50030 (red) and panspecific mouse monoclonal antibody to nuclear pore complexes NBP1-05390 (green). Casein Kinase 1 alpha has a particulate distribution both in the nucleus and the cytoplasm. Blue is the Hoechst DNA stain.](http://images.novusbio.com/fullsize/Casein-Kinase-1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50030-img0003.jpg)
![Immunoprecipitation: Casein Kinase 1 alpha Antibody (PSH04-46) [NBP3-32107] - Casein Kinase 1 alpha was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32107 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32107 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32107 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32107 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 11 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32107_rabbit-casein-kinase-1-alpha-mab-psh04-46-2682024158264.jpg "Immunoprecipitation: Casein Kinase 1 alpha Antibody (PSH04-46) [NBP3-32107] - Casein Kinase 1 alpha was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32107 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32107 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32107 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32107 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 11 seconds; ECL")
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 alpha Antibody (PSH04-46) [NBP3-32107] - Immunocytochemistry analysis of HCT 116 cells labeling Casein Kinase 1 alpha with Rabbit anti-Casein Kinase 1 alpha antibody (NBP3-32107) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 1 alpha antibody (NBP3-32107) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32107_rabbit-casein-kinase-1-alpha-mab-psh04-46-29202410484857.jpg)
![Flow Cytometry: Casein Kinase 1 alpha Antibody (PSH04-46) [NBP3-32107] - Flow cytometric analysis of HCT 116 cells labeling Casein Kinase 1 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32107, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32107_rabbit-casein-kinase-1-alpha-mab-psh04-46-29202416372134.jpg)
![Western Blot: Casein Kinase 1 alpha Antibody (PSH04-46) [NBP3-32107] - Western blot analysis of Casein Kinase 1 alpha on different lysates with Rabbit anti-Casein Kinase 1 alpha antibody (NBP3-32107) at 1/2,000 dilution.Lane 1: HCT 116 cell lysate (20 µg/Lane)Lane 2: HeLa cell lysate (20 µg/Lane)Lane 3: HEK-293 cell lysate (20 µg/Lane)Lane 4: MCF7 cell lysate (20 µg/Lane)Lane 5: Jurkat cell lysate (20 µg/Lane)Lane 6: NIH/3T3 cell lysate (20 µg/Lane)Lane 7: RAW264.7 cell lysate (20 µg/Lane)Lane 8: PC-12 cell lysate (20 µg/Lane)Lane 9: Mouse brain tissue lysate (40 µg/Lane)Lane 10: Rat brain tissue lysate (40 µg/Lane)Predicted band size: 39 kDaObserved band size: 34 kDaExposure time: 15 seconds; ECL;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32107_rabbit-casein-kinase-1-alpha-mab-psh04-46-3920248453528.jpg)
![Western Blot: Casein Kinase 1 alpha Antibody (9O4L9) [NBP3-16807] - Western blot analysis of extracts of various cell lines, using Casein Kinase 1 alpha (Casein Kinase 1 alpha) Rabbit mAb (NBP3-16807) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Casein-Kinase-1-alpha-Antibody-9O4L9-Western-Blot-NBP3-16807-img0002.jpg "Western Blot: Casein Kinase 1 alpha Antibody (9O4L9) [NBP3-16807] - Western blot analysis of extracts of various cell lines, using Casein Kinase 1 alpha (Casein Kinase 1 alpha) Rabbit mAb (NBP3-16807) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Casein Kinase 1 alpha Antibody (9O4L9) [NBP3-16807] - Immunohistochemistry of paraffin-embedded rat liver using Casein Kinase 1 alpha (Casein Kinase 1 alpha) Rabbit mAb (NBP3-16807) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Casein-Kinase-1-alpha-Antibody-9O4L9-Immunohistochemistry-Paraffin-NBP3-16807-img0001.jpg)
![Western Blot: Casein Kinase 1 alpha Antibody (2E2) [NBP1-30165] - Cell lysates of HeLa and MCF7 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Casein Kinase 1 alpha (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/Casein-Kinase-1-alpha-Antibody-2E2-Western-Blot-NBP1-30165-img0002.jpg "Western Blot: Casein Kinase 1 alpha Antibody (2E2) [NBP1-30165] - Cell lysates of HeLa and MCF7 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Casein Kinase 1 alpha (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: Casein Kinase 1 alpha Antibody (2E2) [NBP1-30165] - Analysis of Casein Kinase 1 alpha in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Casein Kinase 1 alpha antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Casein-Kinase-1-alpha-Antibody-2E2-Immunocytochemistry-Immunofluorescence-NBP1-30165-img0006.jpg)
![Western Blot: Casein Kinase 1 alpha Antibody (2E2) [NBP1-30165] - Lane 1.: Jurkat cell lysate. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Caseine kinase 1 alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/Casein-Kinase-1-alpha-Antibody-2E2-Western-Blot-NBP1-30165-img0003.jpg)
![SDS-Page: Recombinant Human Casein Kinase 1 alpha Protein [NBP2-51623]](http://images.novusbio.com/fullsize/Recombinant-Human-Casein-Kinase-1-alpha-Protein-SDS-Page-NBP2-51623-img0002.jpg "SDS-Page: Recombinant Human Casein Kinase 1 alpha Protein [NBP2-51623]")