Casein Kinase 1 alpha Antibody (SR1581) - BSA Free

Western Blot: Casein Kinase 1 alpha Antibody (SR1581) [NBP3-22219] - Western blot analysis using NBP3-22219 in (1) MCF7 cell lysate; (2) NIH/3T3 cell lysate.

Western blot shows lysates of HCT 116 human colorectal carcinoma cell line and Casein Kinase 1 alpha knockdown HCT 116 cell line (KD). Nitrocellulose membrane was probed with Casein Kinase 1 alpha Antibody (SR1581) (Catalog # NBP3-22219) followed by an HRP-conjugated secondary antibody. A specific band was detected for Casein Kinase 1 alpha at approximately 36 kDa (as indicated) in the parental HCT 116 cell line, but is significantly reduced in knockdown HCT 116 cell line. Primary antibody dilution used: 1/500. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

HCT 116 WT and Casein Kinase 1 alpha KD cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Casein Kinase 1 alpha Antibody (SR1581) (Catalog # NBP3-22219) and with an Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KD cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KD cells are outlined with green and magenta dashed line, respectively. Primary antibody dilution used: 1/500. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, Mycoplasma
• Clone: SR1581
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Casein Kinase 1 alpha Antibody (SR1581) - BSA Free Summary

• Description: Novus Biologicals Rabbit Casein Kinase 1 alpha Antibody (SR1581) - BSA Free (NBP3-22219) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human Casein Kinase 1 alpha (Uniprot #: P48729)
• Specificity: Detects endogenous levels of total Casein Kinase 1 alpha
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CSNK1A1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Validated for Immunocytochemistry/Immunofluorescence from YCharOS Inc. (ycharos.com)
  • Knockdown Validated Validated for Knockdown from YCharOS Inc. (ycharos.com)
  • Western Blot 1:500-1:2000
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Casein Kinase 1 alpha Antibody (SR1581) - BSA Free

• Casein Kinase 1 alpha
• casein kinase 1, alpha 1
• casein kinase I isoform alpha
• CK1CKI-alpha
• CSNK1A1
• down-regulated in lung cancer
• EC 2.7.11.1
• HLCDGP1
• PRO2975

Background

Casein kinase I (also designated CKI) and casein kinase II (CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. Casein kinase I family members, which include casein kinase Ialpha, Igamma, Idelta and Iepisilon, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an alpha2beta2 or an alphaalpha'beta2 structure. The a catalytic subunit is stimulated by the beta regulatory subunit, which undergoes autophosphorylation. Casein kinase II activity is high in the cytosol and nucleus of proliferating and differentiating cells. Casein kinase II is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSNK1A1