Carnosine Dipeptidase 1/CNDP1 Antibody (352216) [Unconjugated]

Carnosine Dipeptidase 1/CNDP1 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Carnosine Dipeptidase 1/CNDP1 Monoclonal Antibody (Catalog # MAB2489) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Carnosine Dipeptidase 1/CNDP1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neurons using Mouse Anti-Human Carnosine Dipeptidase 1/CNDP1 Monoclonal Antibody (Catalog # MAB2489) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, IHC, IP, ICC/IF
• Clone: 352216
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Carnosine Dipeptidase 1/CNDP1 Antibody (352216) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Carnosine Dipeptidase 1/CNDP1
  Pro28-His507
  Accession # Q96KN2
• Specificity: Detects human Carnosine Dipeptidase 1/CNDP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CNDP2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CNDP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carnosine Dipeptidase 1/CNDP1 Antibody (352216) [Unconjugated]

• beta-Ala-His dipeptidase
• Carnosinase 1
• carnosine dipeptidase 1 (metallopeptidase M20 family)
• Carnosine Dipeptidase 1
• CN1MGC102737
• CNDP dipeptidase 1
• CNDP1
• CPGL2
• CPGL2MGC142072
• EC 3.4.13.20
• Glutamate carboxypeptidase-like protein 2
• HsT2308
• MGC10825
• Serum carnosinase

Background

The human CNDP1 gene encodes carnosine dipeptidase 1, a member of the M20 family of metalloproteases (1, 2). Also known as X-His dipeptidase, glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1), CNDP1 is a secreted dipeptidase with a narrow specificity for Xaa-His dipeptides including those with Xaa = beta -Ala (carnosine) and Xaa = gamma -aminobutyric acid (homocarnosine), two naturally occurring dipeptides with potential neuroprotective and neurotransmitter fucntions in the brain. In comparison, a closely related protein known as CNDP2, CPGL or CN2, is a cytosolic nonspecific dipeptidase.

1. Teufel, M. et al. (2004) J. Biol. Chem. 278:6521.
2. Bauer, K. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1022, Academic Press, San Diego.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CNDP1
• Uniprot:
  • Q96KN2()