Carm1 Antibody (3H2)

Western Blot: Carm1 Antibody (3H2) [NBP2-37645] - Western blot analysis using CARM1 mouse mAb against MCF-7 (1), Hela (2), NIH/3T3 (3), HL-60 (4), LNcap (5), Jurkat (6), PC-3 (7), Cos7 (8), and PC-12 (9) cell lysate.

Immunocytochemistry/Immunofluorescence: Carm1 Antibody (3H2) [NBP2-37645] - Immunofluorescence analysis of Hela cells using CRAM1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: Carm1 Antibody (3H2) [NBP2-37645] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) using CARM1 mouse mAb with DAB staining.

Flow Cytometry: Carm1 Antibody (3H2) [NBP2-37645] - Flow cytometric analysis of Lovo cells using CARM1 mouse mAb (green) and negative control (purple).

• Reactivity: Hu, Rt, Pm, Pm
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 3H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Carm1 Antibody (3H2) Summary

• Description: Novus Biologicals Mouse Carm1 Antibody (3H2) (NBP2-37645) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Carm1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CARM1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Carm1 Antibody (3H2)

• Carm1
• coactivator-associated arginine methyltransferase 1EC 2.1.1.-
• EC 2.1.1
• histone-arginine methyltransferase CARM1
• PRMT4
• PRMT4EC 2.1.1.125
• Protein arginine N-methyltransferase 4

Background

Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 (PubMed 11724789)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CARM1