CARKL Antibody - BSA Free

Immunoprecipitation: CARKL Antibody [NBP2-61224] - Detection of Human SHPK by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SHPK antibody NBP2-61224 used for IP at 20 ul per reaction. SHPK was also immunoprecipitated by rabbit anti-SHPK antibody NBP2-61223. For blotting immunoprecipitated SHPK, NBP2-61224 was used at 1:100. Detection: Chemiluminescence with an exposure time of 3 minutes.

Product Discontinued

CARKL Antibody - BSA Free Summary

• Description: Western blot of immunoprecipitates performed using ReliaBLOT Reagents and 4-20% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
• Immunogen: 100% Sequence Identity Orangutan between 250 and 300
• Predicted Species: Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SHPK
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 20 ul/mg lysate
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
• Buffer: Tris-buffered Saline containing 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Notes

Western blot of immunoprecipitates performed using ReliaBLOT Reagents and 4-20% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Alternate Names for CARKL Antibody - BSA Free

• Carbohydrate kinase-like protein
• carbohydrate kinase-like
• CARKLEC 2.7.1.14
• sedoheptulokinase
• SHKFLJ32478

Background

CARKL is encoded by this gene has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. Sequence variation between this novel gene and known carbohydrate kinases suggests the possibility of a different substrate, cofactor or changes in kinetic properties distinguishing it from other carbohydrate kinases. The gene resides in a region commonly deleted in cystinosis patients, suggesting a role as a modifier for the cystinosis phenotype. The genomic region is also rich in Alu repetitive sequences, frequently involved in chromosomal rearrangements. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SHPK