CTNS Antibody (5G6) - Azide and BSA Free

Western Blot: CTNS Antibody (5G6) [H00001497-M09] - Analysis of CTNS expression in human liver.

Western Blot: CTNS Antibody (5G6) [H00001497-M09] - Analysis of CTNS expression in PC-12.

Western Blot: CTNS Antibody (5G6) [H00001497-M09] - Analysis of CTNS expression in HepG2.

Western Blot: CTNS Antibody (5G6) [H00001497-M09] - Analysis of CTNS expression in Raw 264.7.

ELISA: CTNS Antibody (5G6) [H00001497-M09] - Detection limit for recombinant GST tagged CTNS is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clone: 5G6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CTNS Antibody (5G6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CTNS (NP_004928, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MIRNWLTIFILFPLKLVEKCESSVSLTVPPVVKLENGSSTNVSLTLRPPLNATLVITFEITFRSKNITILELPDEVVVPPGVTNSSFQVTSQNVGQLTVY
• Specificity: CTNS - cystinosis, nephropathic
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CTNS
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CTNS Antibody (5G6) - Azide and BSA Free

• CTNS-LSB
• cystinosin
• cystinosis, nephropathic
• PQLC4

Background

The CTNS gene encodes a seven-transmembrane domain protein that functions to transport cystine out of lysosomes. Itsactivity is driven by the H+ electrochemical gradient of the lysosomal membrane. Mutations in this gene causecystinosis, a lysosomal storage di

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CTNS Antibody (H00001497-M09)(3)

Publications using H00001497-M09

• Brasell EJ, Chu L, El Kares R et al. The aminoglycoside geneticin permits translational readthrough of the CTNS W138X nonsense mutation in fibroblasts from patients with nephropathic cystinosis. Pediatr Nephrol. 2018-11-09 [PMID: 30413946]
• McEvoy B, Sumayao R, Slattery C et al. Cystine accumulation attenuates insulin release from the pancreatic beta-cell due to elevated oxidative stress and decreased ATP levels. J Physiol. 2015-10-20 [PMID: 26482480]
• Bellomo F, Corallini S, Pastore A et al. Modulation of CTNS gene expression by intracellular thiols. Free Radic Biol Med;48(7):865-72. 2010-04-01 [PMID: 20079424]

Bioinformatics

• Gene Symbol: CTNS
• Entrez:
  • 1497(Human)
• OMIM:
  • 219750(Human)
• Uniprot:
  • NP_004928(Human)