CARD8 Antibody

Product Discontinued

CARD8 Antibody Summary

• Immunogen: Antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARD8. The immunogen is located within the last 50 amino acids of CARD8.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CARD8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1-2 ug/ml
• Application Notes: CARD8 antibody can be used for detection of CARD8 by Western blot at 1 -2 ug/mL. An approximate 45 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL.
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CARD8 Antibody

• Apoptotic protein NDPP1
• CARD inhibitor of NF-kappaB-activating ligands
• CARDINALFLJ18121
• CARD-inhibitor of NF-kappa-B-activating ligand
• caspase recruitment domain family, member 8
• caspase recruitment domain-containing protein 8
• DACAR
• Dakar
• KIAA0955DKFZp779L0366
• NDPP
• NDPP1MGC57162
• TUCANFLJ18119
• Tumor up-regulated CARD-containing antagonist of CASP9
• tumor up-regulated CARD-containing antagonist of caspase nine

Background

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis, and caspase-1 and its related proteins ICEBERG and pesudo-ICE. Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein, it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-kappaB activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CARD8
• Uniprot:
  • AAG50014(Human)