CARD8 Antibody

Images

 
Western Blot: CARD8 Antibody [NBP1-77220] - Analysis of CARD8 expression in human lung tissue lysate with CARD8 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: CARD8 Antibody [NBP1-77220] - K562 cells with CARD8 antibody at 10 ug/ml.
Western Blot: CARD8 Antibody [NBP1-77220] - Analysis of CARD8 expression in K562 cell lysate with anti-CARD8 using NBP1-77220 at (A) 2 and (B) 4 ug /ml.
Immunofluorescence: CARD8 Antibody [NBP1-77220] - Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 ug/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, ICC, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CARD8 Antibody Summary

Immunogen
Antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARD8. The immunogen is located within the last 50 amino acids of CARD8.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CARD8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunocytochemistry
  • Immunofluorescence 20 ug/ml
Application Notes
CARD8 antibody can be used for detection of CARD8 by Western blot at 1 -2 ug/mL. An approximate 45 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL.
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
CARD8 Peptide (NBP1-77220PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CARD8 Antibody

  • Apoptotic protein NDPP1
  • CARD inhibitor of NF-kappaB-activating ligands
  • CARDINALFLJ18121
  • CARD-inhibitor of NF-kappa-B-activating ligand
  • caspase recruitment domain family, member 8
  • caspase recruitment domain-containing protein 8
  • DACAR
  • DAKAR
  • KIAA0955DKFZp779L0366
  • NDPP
  • NDPP1MGC57162
  • TUCANFLJ18119
  • Tumor up-regulated CARD-containing antagonist of CASP9
  • tumor up-regulated CARD-containing antagonist of caspase nine

Background

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis, and caspase-1 and its related proteins ICEBERG and pesudo-ICE. Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein, it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-kappaB activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Isotype Controls

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Bioinformatics

Gene Symbol CARD8
Uniprot