Human Carboxypeptidase B1/CPB1 ELISA Kit (Colorimetric)

ELISA: Human Carboxypeptidase B1/CPB1 ELISA Kit (Colorimetric) [NBP3-17990] - Standard Reference Curve

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum
• Standard Curve Range: 6.25-400 pg/mL (example only; lot dependent)
• Sensitivity: 5.30 pg/mL

Human Carboxypeptidase B1/CPB1 ELISA Kit (Colorimetric) Summary

• Specificity: Recognizes both recombinant and natural Human Ferritin light chain
• Standard Curve Range: 6.25-400 pg/mL (example only; lot dependent)
• Sensitivity: 5.30 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 9.7
• Spike Recovery: 83 -93%
• Sample Volume: [Sample Volume]
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CPB1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Carboxypeptidase B1/CPB1 ELISA Kit (Colorimetric)

• carboxypeptidase B
• carboxypeptidase B1 (tissue)
• Carboxypeptidase B1
• CPB
• CPB1
• DKFZp779K1333
• EC 3.4.17
• EC 3.4.17.2
• Pancreas-specific protein
• PASP
• PCPB
• procarboxypeptidase B

Background

Carboxypeptidase catalyzes the hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal position in polypeptides. It occurs as a zymogen in the pancreas in most vertebrates. This reagent is suited to enable an immunological appraoch to the study of protein and peptide structure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Carboxypeptidase B1/CPB1 ELISA Kit (NBP3-17990). (Showing 1 - 2 of 2 FAQ).

1. I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?
   • Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.
2. I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit .
   • Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

Bioinformatics

• Gene Symbol: CPB1