Carboxypeptidase B1/CPB1 Antibody

Western Blot: Carboxypeptidase B1/CPB1 Antibody [H00001360-B01P] - Analysis of CPB1 expression in human pancreas.

Western Blot: Carboxypeptidase B1/CPB1 Antibody [H00001360-B01P] - Analysis of CPB1 expression in transfected 293T cell line by CPB1 polyclonal antibody. Lane 1: CPB1 transfected lysate(45.87 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

Carboxypeptidase B1/CPB1 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: CPB1 (NP_001862.2, 1 a.a. - 417 a.a.) full-length human protein. MLALLVLVTVALASAHHGGEHFEGEKVFRVNVEDENHINIIRELASTTQIDFWKPDSVTQIKPHSTVDFRVKAEDTVTVENVLKQNELQYKVLISNLRNVVEAQFDSRVRATGHSYEKYNKWETIEAWTQQVATENPALISRSVIGTTFEGRAIYLLKVGKAGQNKPAIFMDCGFHAREWISPAFCQWFVREAVRTYGREIQVTELLDKLDFYVLPVLNIDGYIYTWTKSRFWRKTRSTHTGSSCIGTDPNRNFDAGWCEIGASRNPCDETYCGPAAESEKETKALADFIRNKLSSIKAYLTIHSYSQMMIYPYSYAYKLGENNAELNALAKATVKELASLHGTKYTYGPGATTIYPAAGGSDDWAYDQGIRYSFTFELRDTGRYGFLLPESQIRATCEETFLAIKYVASYVLEHLY
• Specificity: CPB1 - carboxypeptidase B1 (tissue),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: CPB1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: It has been used for WB.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Carboxypeptidase B1/CPB1 Antibody

• carboxypeptidase B
• carboxypeptidase B1 (tissue)
• Carboxypeptidase B1
• CPB
• CPB1
• DKFZp779K1333
• EC 3.4.17
• EC 3.4.17.2
• Pancreas-specific protein
• PASP
• PCPB
• procarboxypeptidase B

Background

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Carboxypeptidase B1/CPB1 Antibody (H00001360-B01P). (Showing 1 - 2 of 2 FAQ).

1. I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?
   • Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.
2. I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit .
   • Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

Bioinformatics

• Gene Symbol: CPB1