Carboxypeptidase B1/CPB1 Antibody (438806)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clone
438806
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Carboxypeptidase B1/CPB1 Antibody (438806) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase B1/CPB1
His16-Tyr417
Accession # P15086
Specificity
Detects human Carboxypeptidase B1/CPB1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CPA1, rhCPA2, rhCPE, recombinant mouse (rm) CPA4, and rmCPB1 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CPB1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB2897 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carboxypeptidase B1/CPB1 Antibody (438806)

  • carboxypeptidase B
  • carboxypeptidase B1 (tissue)
  • Carboxypeptidase B1
  • CPB
  • CPB1
  • DKFZp779K1333
  • EC 3.4.17
  • EC 3.4.17.2
  • Pancreas-specific protein
  • PASP
  • PCPB
  • procarboxypeptidase B

Background

Carboxypeptidase B1, encoded by the CPB1 gene, specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg (1). Expressed mainly in pancreas, CPB1 is a useful serum marker for acute pancreatitis (2). The deduced amino acid (aa) sequence of human CPB1 consists of a signal peptide (aa 1-15), a pro region (aa 16-110), and a mature chain (aa 111-417). Human CPB1 shares 98%, 84%, 76%, 75%, and 84% aa sequence identity with chimpanzee, dog, rat, mouse and chicken CPB1, respectively.

  1. Aviles, F.X. and J. Vendrell (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) p. 831, Academic Press, San Diego.
  2. Yamamoto, K.K. et al. (1992) J. Biol. Chem. 267:2575.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Carboxypeptidase B1/CPB1 Antibody (MAB2897)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Carboxypeptidase B1/CPB1 Antibody (MAB2897). (Showing 1 - 2 of 2 FAQ).

  1. I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?
    • Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.
  2. I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit .
    • Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Carboxypeptidase B1/CPB1 Antibody (MAB2897)

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Diseases for Carboxypeptidase B1/CPB1 Antibody (MAB2897)

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Bioinformatics

Gene Symbol CPB1
Uniprot