Carboxylesterase 1/CES1 Antibody

Western Blot: CES1 Antibody [NBP1-00167] - Analysis of CES1 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-00167 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: CES1 Antibody [NBP1-00167] - (2.5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Carboxylesterase 1/CES1 Antibody Summary

• Description: Novus Biologicals Goat Carboxylesterase 1/CES1 Antibody (NBP1-00167) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NTQAAQKLKDKE corresponding to internal region (near C-Terminus) according to NP_001020366.1, NP_001020365.1, NP_001257.4.
• Specificity: This antibody is expected to recognise all three reported isoforms (NP_001020366.1; NP_001020365.1; NP_001257.4).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CES1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2 - 3 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 55-60 kDa band observed in human liver and Ileum lysates (calculated MW of 62.6 kDa band according to NP_001020366.1). In transfected HEK293 transiently expressing CES1 a band of approx. 65 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human Lung shows staining of lymphocytes in the vasculature.
• Control:

  Carboxylesterase 1/CES1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Carboxylesterase 1/CES1 Antibody

• ACAT
• acyl coenzyme A:cholesterol acyltransferase
• Acyl-coenzyme A:cholesterol acyltransferase
• Brain carboxylesterase hBr1
• carboxylesterase 1 (monocyte/macrophage serine esterase 1)
• Carboxylesterase 1
• carboxylesterase 2 (liver)
• CEH
• CES1
• Ces-1
• CES1A2
• CES2EC 3.1.1.1
• cholesteryl ester hydrolase
• Cocaine carboxylesterase
• EC 3.1.1
• Egasyn
• HMSE1
• HMSECES1A1
• human monocyte/macrophage serine esterase 1
• liver carboxylesterase 1
• MGC117365
• Monocyte/macrophage serine esterase
• PCE-1
• REH
• Retinyl ester hydrolase
• Serine esterase 1
• SES1
• Ses-1
• SES1TGH
• TGH
• Triacylglycerol hydrolase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CES1
• Entrez:
  • 1066(Human)
• Uniprot:
  • NP_001020366.1(Human)