Carbonic Anhydrase XIV/CA14 Antibody (312908)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 312908
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Carbonic Anhydrase XIV/CA14 Antibody (312908) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase XIV/CA14
  Gly19-Met290
  Accession # Q9ULX7
• Specificity: Detects human Carbonic Anhydrase XIV/CA14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross‑reactivity with recombinant mouse CA14, 10% cross‑reactivity with recombinant human (rh) CA5A, and no cross-reactivity with rhCA1, 2, 3, 4, 6, 7, 8, 9, 10, 12, or 13 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CA14
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase XIV/CA14 Antibody (312908)

• CA14
• Carbonate dehydratase XIV
• Carbonic Anhydrase XIV
• carbonic anhydrase XIVcarbonic anhydrase 14
• carbonic dehydratase
• CAXiV
• CA-XIV
• EC 4.2.1.1

Background

Carbonic Anhydrase 14 (CA14) has a C-terminal transmembrane domain (aa 291 - 311) and a short cytoplasmic tail (aa 312 - 337). As a functioning enzyme of the CA family, it catalyzes the reversible hydration of CO2. CA14 is highly expressed in all parts of the central nervous system with lower expression in adult liver, heart, small intestine, colon, kidney, urinary bladder and skeletal muscle. The rhCA14 corresponds to the 272 aa ectodomain that has a predicted molecular mass of approximately 32 kDa and an apparent molecular mass of approximately 43 kDa in SDS-PAGE. The amino acid sequence of the human ectodomain shares 98%, 86%, 84%, and 83% identity with that of chimpanzee, bovine/dog, rat and mouse, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CA14
• Uniprot:
  • Q9ULX7()