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Human Carbonic Anhydrase XII/CA12 ELISA Kit (Colorimetric)

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Human Carbonic Anhydrase XII/CA12 ELISA Kit (Colorimetric) - Standard Curve Reference
Human Carbonic Anhydrase XII/CA12 ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

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Human Carbonic Anhydrase XII/CA12 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CA12 in samples. No significant cross-reactivity or interference between Human CA12 and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.9%
Intra-Assay
CV% < 5.68%
Spike Recovery
86-104%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CA12

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Wash Buffer (25×)
  3. Concentrated HRP Conjugate (100×)
  4. Certificate of Analysis
  5. Concentrated Biotinylated Detection Ab (100×)
  6. Product Description
  7. Plate Sealer
  8. Reference Standard
  9. Reference Standard & Sample Diluent
  10. HRP Conjugate Diluent
  11. Micro ELISA Plate(Dismountable)
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Human Carbonic Anhydrase XII/CA12 ELISA Kit (Colorimetric)

  • CA12
  • Carbonate dehydratase XII
  • carbonic anhydrase 12
  • Carbonic Anhydrase XII
  • carbonic anhydrase XIICAXII
  • carbonic dehydratase
  • CA-XII
  • EC 4.2.1.1
  • FLJ20151
  • HsT18816
  • Tumor antigen HOM-RCC-3.1.3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Immunohistochemistry: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Images showing Carbonic Anhydrase IX/CA9 expression, pimonidazole, apoptosis and proliferation in SCCNij202.Carbonic Anhydrase IX/CA9 is expressed in hypoxic regions as assessed by pimonidazole staining (A), but is also observed in non-pimonidazole areas (B). Yet, most of Carbonic Anhydrase IX/CA9 is expressed in pimonidazole positive regions (C). Proliferation (BrdUrd) and apoptosis (caspase-3) in relation to Carbonic Anhydrase IX/CA9 expression are shown in figure D and E. Red, Carbonic Anhydrase IX/CA9; Green, pimonidazole (A-B), BrdUrd (D) or caspase-3 (E); Yellow, overlap of Carbonic Anhydrase IX/CA9 (red) and pimonidazole (green); Light blue, vessels. Magnification 100x. Scale bars represent 100 um. Closed circles represent Carbonic Anhydrase IX/CA9 expression in pimonidazole positive regions; open circles represent Carbonic Anhydrase IX/CA9 expression in pimonidazole negative regions. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0108068), licensed under a CC-BY licence.
Dual RNAscope ISH-IHC: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Formalin-fixed paraffin-embedded tissue sections of human stomach were probed for Carbonic Anhydrase IX/CA9 mRNA (ACD RNAScope Probe, catalog # 559348; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus catalog # NB100-417) at 1:1000 dilution with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.
Western Blot: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis on various human cell lysates. Specific bands were detected for Carbonic Anhydrase IX/CA9 in HEK-293 and A498 cell lines at a molecular weight of approximately 50 kDa. WB image submitted by a verified customer review.
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(201 Publications)
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Applications: WB, Simple Western, ChIP, ELISA, Flow, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, MiAr, Dual ISH-IHC, KO
Carbonic Anhydrase
II/CA2 Antibody
NB600-919
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Differential expression of CAIX and CAXII in patient-derived tumor grafts. Frozen tissue samples from PDX tumors were homogenized in RIPA buffer containing protease inhibitor. Western blots were probed with antibodies for CAIX, CAXII, CAII, ER, E-cadherin, actin, and GAPDH. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0199476) licensed under a CC-BY licence.
Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Staining in nucleus and cytoplasm of proximal and distal tubules, bowman's capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 ug/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3'3-diamino-benzidine substrate (DAB) or WARP RED.
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Detection of Carbonic Anhydrase II in mouse embryonic whole cell lysate. Image provided via product review by verified customer.
(3 Reviews)
(8 Publications)
Species: Hu, Mu
Applications: WB, ELISA, IHC, IHC-Fr, IHC-P
Carbonic Anhydrase
XIII/CA13 Antibody
NBP2-92743
Western Blot: Carbonic Anhydrase XIII/CA13 Antibody [NBP2-92743] - Analysis of extracts of various cell lines, using Carbonic Anhydrase XIII/CA13 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunohistochemistry-Paraffin: Carbonic Anhydrase XIII/CA13 Antibody [NBP2-92743] - Paraffin-embedded mouse kidney using Carbonic Anhydrase XIII/CA13.
Immunohistochemistry-Paraffin: Carbonic Anhydrase XIII/CA13 Antibody [NBP2-92743] - Paraffin-embedded human breast cancer using Carbonic Anhydrase XIII/CA13.
Species: Hu, Mu
Applications: WB, IHC
HIF-1 alpha
Antibody (H1alpha67)
NB100-105
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. WB image submitted by a verified customer review.
Knockout Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog # NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using a NorthernLights (TM) 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1 alpha-deficient (HIF-1 alpha KO) mice were differentiated under IL-22-skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1 alpha and Lamin B1 levels were analyzed by Western blot. Image from verified customer review.
(40 Reviews)
(868 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca, Fe, Ma, Pm, Pm, Rb, Sh, Xp
Applications: WB, ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, PLA, TCS, KD, KO, LA
Carbonic Anhydrase
IV/CA4 Antibody (3104 ...
MAB2186
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human lung tissue. PVDF membrane was probed with 2&amp;nbsp;µg/mL of Mouse Anti-Human Carbonic Anhydrase&amp;nbsp;IV/CA4 Monoclonal Antibody (Catalog # MAB2186) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog&amp;nbsp;# HAF007). A specific band was detected for Carbonic Anhydrase&amp;nbsp;IV/CA4 at approximately&amp;nbsp;35&amp;nbsp;kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
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Species: Hu
Applications: WB, IHC, IP
TFF1/pS2 Antibody
NBP1-90813
Western Blot: TFF1/pS2 Antibody [NBP1-90813] - Analysis in ysate from HEK293 cells. Image from a verified customer review.
Immunohistochemistry-Paraffin: TFF1/pS2 Antibody [NBP1-90813] - Staining in human stomach and liver tissues using anti-TFF1 antibody. Corresponding TFF1 RNA-seq data are presented for the same tissues.
Western Blot: TFF1/pS2 Antibody [NBP1-90813] - Analysis in control (vector only transfected HEK293T lysate) and TFF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
(1 Review)
(4 Publications)
Species: Hu
Applications: WB, IHC, IHC-P
Von Hippel Lindau
Antibody
NB100-41384
Western Blot: Von Hippel Lindau Antibody [NB100-41384] - (0.01ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Von Hippel Lindau Antibody [NB100-41384] - (5ug/ml) staining of Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA: Von Hippel Lindau Antibody [NB100-41384] - (1.5ug/ml) as the reporter with VHL antibody as the capture rabbit antibody (2.5ug/ml).
(1 Publication)
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Applications: WB, ELISA, IHC, IHC-P, PEP-ELISA, S-ELISA
u-Plasminogen
Activator/Urokinase [ ...
1310-SE
Recombinant Human u-Plasminogen Activator/Urokinase (Catalog&amp;nbsp;# 1310-SE) is measured by its ability to cleave a peptide substrate,N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC).
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p53 Antibody (PAb
240)
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
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Transgelin/TAGLN/SM22
NB600-507
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507] - Effects of ROCK1 and ZIPK knockdown on IL-1beta protein expression.IL-1beta protein expression in control cells and cells transfected with ZIPK or ROCK1 siRNA was examined by western blotting. Two representative western blots showing the levels of IL-1beta in control UASMC and in UASMC 48 h after transfection with siRNAs to ZIPK or ROCK1. Two loading controls were used: alpha-actin and SM-22. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0116969) licensed under a CC-BY licence.
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507] - Staining of Testes lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
(1 Review)
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VEGF [HRP]
DVE00
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Progesterone
R/NR3C3 Antibody (4E9
H00005241-M08
Western Blot: Progesterone R/NR3C3 Antibody (4E9) [H00005241-M08] - Analysis of PGR expression in MCF-7 (Cat # L046V1).
Immunocytochemistry/Immunofluorescence: Progesterone R/NR3C3 Antibody (4E9) [H00005241-M08] - Analysis of monoclonal antibody to PGR on MCF-7 cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (4E9) [H00005241-M08] - Analysis of monoclonal antibody to PGR on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 3 ug/ml
(1 Publication)
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
Carbonic Anhydrase
XII/CA12 Antibody [Un ...
AF2345
Carbonic Anhydrase XII/CA12 Antibody [Unconjugated]
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NB600-1182
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of a FFPE human breast carcinoma tissue section using 1:100 dilution of EpCAM/TROP1 antibody (clone AUA1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This EpCAM antibody generated an expected immunostaining of EpCAM (CD326) protein in the membranes of the cancer cells and the inter-cellular spaces. The tumor stroma and the stromal cells did not show EpCAM immunopositivity.
Western Blot: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Detection of EpCAM/CD326 in two Human mammary tumor cell lines, MDA-MB-231 (mesenchyme-like) and MCF-7 (epithelium-like). Dilution: 1:500 in PBS with 5% BSA. Secondary Ab: anti-Mouse IgG 1:5,000. This image was submitted via customer Review.
Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-EpCAM/TROP1 (AUA1) conjugated to Alexa Fluor 488 [NB600-1182AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.
(1 Review)
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Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
(1 Review)
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Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
CFTR Antibody
(24-1) [Unconjugated] ...
MAB25031
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8&amp;nbsp;µg/mL Mouse Anti-Human CFTR C‑Terminus Monoclonal Antibody (Catalog&amp;nbsp;# MAB25031) overnight at 4&amp;nbsp;°C. Tissue was stained with the Anti-Mouse HRP-AEC Cell &amp;amp; Tissue Staining Kit (red; Catalog&amp;nbsp;# CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse&amp;nbsp;Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the Anti-Mouse HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog&amp;nbsp;# CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(11 Reviews)
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Applications: WB, IHC, IP
ER alpha/NR3A1
Antibody (33)
NB300-560
Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).
Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).
(1 Review)
(6 Publications)
Species: Hu, Mu, Rt, Av, Bv, Ma, Pm
Applications: WB, IHC, IHC-Fr, IHC-P, IF
HNF-3 alpha/FoxA1
Antibody (3B3NB)
NBP2-45354
Western Blot: HNF-3 alpha/FoxA1 Antibody (3B3NB) [NBP2-45354] - Analysis of FOXA1 (3B3NB) in partial recombinant FOXA1 protein and PC3 lysate, respectivetly.
Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B3NB) [NBP2-45354] - FOXA1 (3B3NB) antibody was tested in HepG2 cells at a 1:25 dilution against DyLight 488 (Green) using a 40X objective. Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respective
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (3B3NB) [NBP2-45354] - Analysis of FoxA1 in human prostate.
(1 Publication)
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Vimentin Antibody
NB300-223
Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5000 in green, revealing multiple bands around 280 kDa that correspond to full length MAP2A/2B isotypes, and ~70 kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Comparative analysis of epithelial and mesenchymal markers expressions. Fluorescence immunocytochemistry staining for E-cadherin (green), N-cadherin (red) and vimentin (cyan) was performed on esophageal cancer cells under static and microfluidic flow conditions at different time points. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep38221), licensed under a CC-BY licence.
Immunohistochemistry: Vimentin Antibody [NB300-223] - Increased numbers of differentiating NSPCs in SVZ of G93A-SOD1 brain. Confocal microscopy images of dorsal and ventral regions of SVZ in G93A-SOD1 and Wt-SOD1 brain at postnatal week 18, stained for nestin (green), GFAP (red), and vimentin (blue). Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (http://www.molecularbrain.com/content/8/1/5), licensed under a CC-BY licence.
(6 Reviews)
(44 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Human Carbonic
Anhydrase XII/CA12 EL ...
NBP2-70053
Human Carbonic Anhydrase XII/CA12 ELISA Kit (Colorimetric)
Species: Hu
Applications: ELISA

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Bioinformatics

Gene Symbol CA12