Carbonic Anhydrase IV/CA4 Antibody [Alexa Fluor™ Plus 594]

• Reactivity: Hu
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 594

Carbonic Anhydrase IV/CA4 Antibody [Alexa Fluor™ Plus 594] Summary

• Specificity: Detects human Carbonic Anhydrase IV/CA4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross‑reactivity with recombinant mouse CA4 is observed and less than 5% cross-reactivity with recombinant human CA1, 2, 3, 8, 9, 10, 12, and 13 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase IV/CA4 Antibody [Alexa Fluor™ Plus 594]

• CA4
• CAIV
• CA-IV
• Car4
• Carbonate dehydratase IV
• carbonic anhydrase 4
• Carbonic Anhydrase IV
• carbonic anhydrase IVRP17
• carbonic dehydratase IV
• EC 4.2.1.1
• retinitis pigmentosa 17 (autosomal dominant)
• RP17

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA4 is a GPI‑anchored membrane enzyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and of proximal renal tubules. It functions as the principal CO₂ taste sensor (2). In addition, a genetic mutation (Arg 14 to Trp in the signal peptide) of CA4 was found to cosegregate with the RP17 form of retinitis pigmentosa in two large families and was not found in 36 unaffected family members or 100 controls (3).

1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
2. Chandrashekar, J. et al. (2009) Science 326:443.
3. Rebello, G. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6617.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.