Carbonic Anhydrase II/CA2 Antibody (322706) [Unconjugated]

Western blot shows lysates of human kidney tissue, Caki-2 human clear cell carcinoma epithelial cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse Carbonic Anhydrase II/ CA2 Monoclonal Antibody (Catalog # MAB2184) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Carbonic Anhydrase II/CA2 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Carbonic Anhydrase II/CA2 was detected in immersion fixed paraffin-embedded sections of human descending colon (left panel) and ileum (right panel) using Rat Anti-Human/Mouse Carbonic Anhydrase II/CA2 Monoclonal Antibody (Catalog # MAB2184) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue).

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: 322706
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Carbonic Anhydrase II/CA2 Antibody (322706) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Carbonic Anhydrase II/CA2
  Ser2-Lys260
  Accession # P00918
• Specificity: Detects human Carbonic Anhydrase II/CA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CA-1, -3, -4, -5A, -5B, -6, -7, -8, or -9 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CA2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.5-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase II/CA2 Antibody (322706) [Unconjugated]

• CA2
• CAII
• CA-IIEC 4.2.1.1
• Car2
• Carbonate dehydratase II
• carbonic anhydrase 2
• carbonic anhydrase B
• Carbonic anhydrase C
• Carbonic Anhydrase II
• carbonic anhydrase IICAC
• carbonic dehydratase

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in the CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in the clinic, as nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification (2).

1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
2. Shah, G.N. et al. (2004) Hum. Mutat. 24:272.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Carbonic Anhydrase II/CA2 Antibody (MAB2184)(5)

Publications using MAB2184

• Transient upregulation of procaspase-3 during oligodendrocyte fate decisions [PMID: 39837665] (Immunohistochemistry, Mouse)
• J Hou, Y Zhou, Z Cai, M Terekhova, A Swain, PS Andhey, RM Guimaraes, A Ulezko Ant, T Qiu, S Sviben, G Strout, JAJ Fitzpatric, Y Chen, S Gilfillan, DH Kim, SJ Van Dyken, MN Artyomov, M Colonna Transcriptomic atlas and interaction networks of brain cells in mouse CNS demyelination and remyelination Cell Reports, 2023-03-21;42(4):112293. 2023-03-21 [PMID: 36952346] (IHC, Mouse)
• W Na, EJ Lee, MK Kang, YH Kim, DY Kim, H Oh, SI Kim, SY Oh, YH Kang Aesculetin Inhibits Osteoclastic Bone Resorption through Blocking Ruffled Border Formation and Lysosomal Trafficking Int J Mol Sci, 2020-11-13;21(22):. 2020-11-13 [PMID: 33203061] (Western Blot, Human)
• EJ Lee, MK Kang, YH Kim, DY Kim, H Oh, SI Kim, SY Oh, W Na, YH Kang Coumarin Ameliorates Impaired Bone Turnover by Inhibiting the Formation of Advanced Glycation End Products in Diabetic Osteoblasts and Osteoclasts Biomolecules, 2020-07-15;10(7):. 2020-07-15 [PMID: 32679814] (Western Blot, Mouse)
• Y Zhou, WM Song, PS Andhey, A Swain, T Levy, KR Miller, PL Poliani, M Cominelli, S Grover, S Gilfillan, M Cella, TK Ulland, K Zaitsev, A Miyashita, T Ikeuchi, M Sainouchi, A Kakita, DA Bennett, JA Schneider, MR Nichols, SA Beausoleil, JD Ulrich, DM Holtzman, MN Artyomov, M Colonna Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and TREM2-independent cellular responses in Alzheimer's disease Nat. Med., 2020-01-13;26(1):131-142. 2020-01-13 [PMID: 31932797] (IHC, Mouse)

Bioinformatics

• Gene Symbol: CA2
• Uniprot:
  • P00918()