Carbonic Anhydrase I/CA1 Antibody - BSA Free

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free

Carbonic Anhydrase I/CA1 Antibody - BSA Free Summary

• Description: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Carbonic Anhydrase I [Human Erythrocytes]
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Carbonic Anhydrase I/CA1/CA1 [Human Erythrocytes] (Uniprot: P00915)
• Specificity: Cross reactivity against Carbonic Anhydrase I/CA1 from other tissues and species may occur but have not been specifically determined.
• Isotype: Serum
• Clonality: Polyclonal
• Host: Goat
• Gene: CA1
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:100000
  • Western Blot 1:2000-1:10000
• Application Notes: This product has been assayed against 1.0 ug of Carbonic Anhydrase I [Human Erythrocytes] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 is suggested for this product.
• Control:

  Carbonic Anhydrase I/CA1 Overexpression Lysate

Reactivity Notes

Cross reactivity against Carbonic Anhydrase I/CA1 from other tissues and species may occur but have not been specifically determined.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Delipidation and Defibrination

Alternate Names for Carbonic Anhydrase I/CA1 Antibody - BSA Free

• CA1
• CA-I
• Car1
• Carbonate dehydratase I
• carbonic anhydrase 1
• Carbonic anhydrase B
• Carbonic Anhydrase I
• carbonic anhydrase ICAB
• carbonic dehydratase
• EC 4.2.1.1

Background

Carbonic anhydrase performs an important role in respiration by facilitating transfer of CO2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CA1
• Entrez:
  • 759(Human)
• Uniprot:
  • P00915()
  • P00915()
  • P00915()
  • P00915()