Cannabinoid R1/CB1/CNR1 Antibody

Immunocytochemistry/Immunofluorescence: Cannabinoid R1/CB1/CNR1 Antibody [NB300-552] - Immunlocalization of rat CB1 transfected into AtT20 cells.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Cannabinoid R1/CB1/CNR1 Antibody Summary

• Immunogen: A fusion protein containing the first 99 amino acid residues from human CB1.
• Localization: Integral membrane protein
• Specificity: Detects CB1 from human and rat tissues as well as transfected rat CB1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CNR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1:250
• Application Notes: WB: Detects an approx. 60 kDa protein representing CB1 from rat brain homogenate. ICC: Staining of CB1 in AtT20 cells transfected with the rat CB1 gene yields a pattern consistent with plasma membrane staining.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA and 30% glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cannabinoid R1/CB1/CNR1 Antibody

• CANN6
• CANN6CB1R
• Cannabinoid R1
• cannabinoid receptor 1 (brain)
• CannabinoidR1
• CB1
• CB1A
• CB1K5
• CB1R
• CB-R
• CB-RCB1cannabinoid receptor 1
• central cannabinoid receptor
• CNR
• CNR1
• SKR6R

Background

Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P / Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CNR1
• Entrez:
  • 1268(Human)
  • 25248(Rat)