Canine IL-8/CXCL8 Quantikine ELISA Kit

• Reactivity: Ca
• Applications: ELISA
• Conjugate: HRP

Canine IL-8/CXCL8 Quantikine ELISA Kit Summary

• Background: The Quantikine Canine IL-8 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure canine IL-8 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant canine IL-8 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural canine IL-8 sho...wed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring canine IL-8.
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• Specificity: Natural and recombinant canine IL-8. This assay also recognizes the 74 and 79 amino acid variants of recombinant canine IL-8.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CXCL8

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Canine IL-8/CXCL8 Quantikine ELISA Kit

• 3-10C
• AMCF-I
• C-X-C motif chemokine 8
• CXCL8
• CXCL8SCYB8
• Emoctakin
• GCP1
• GCP-1TSG-1
• IL8
• IL-8
• interleukin 8
• K60
• LAI
• LECT
• LUCT
• LYNAP
• MDNCF
• MDNCFb-ENAP
• member 8
• MONAP
• MONAPGCP1
• NAF
• NAP1
• NAP-1NAP1
• NCF
• Neutrophil-activating protein 1
• Protein 3-10C
• T cell chemotactic factor
• T-cell chemotactic factor
• TCF
• TSG1

Background

Interleukin-8 (IL-8), also known as CXCL8, is a chemokine that is upregulated at sites of inflammation where it promotes neutrophil infiltration and activation. IL-8 can form homodimers and heterodimers with CXCL4/PF4. Its bioactivity is regulated by proteolytic truncations, citrullination, and the decoy receptor DARC. IL-8 signals through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines. IL-8 also binds to Serpin A1/alpha-1 Antitrypsin which prevents IL-8 interaction with CXCR1.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CXCL8/IL-8 (CA8000)(5)

Publications using CA8000

• H Suzuki, N Oshima, T Watari Effect of modified ultrafiltration on cytokines and hemoconcentration in dogs undergoing cardiopulmonary bypass J Vet Med Sci, 2020-10-05;0(0):. 2020-10-05 [PMID: 33012753] (Canine)
• D Santoro, K Ahrens, R Vesny, C Navarro, H Gatto, R Marsella Evaluation of the in vitro effect of Boldo and Meadowsweet plant extracts on the expression of antimicrobial peptides and inflammatory markers in canine keratinocytes Res. Vet. Sci., 2017-05-17;115(0):255-262. 2017-05-17 [PMID: 28549300] (Canine)
• L Leclerc, C Thorin, J Flanagan, V Biourge, S Serisier, P Nguyen Higher neonatal growth rate and body condition score at 7�months are predictive factors of obesity in adult female Beagle dogs BMC Vet. Res., 2017-04-13;13(1):104. 2017-04-13 [PMID: 28407742] (Canine)
• Naoaki Matsuki CC chemokine ligand 2 and CXC chemokine ligand 8 as neutrophil chemoattractant factors in canine idiopathic polyarthritis Vet. Immunol. Immunopathol., 2016-09-23;182(0):52-58. 2016-09-23 [PMID: 27863550] (Canine)
• J Chen, G Chen, D Xiao, W Peng, G Yu, Y Lin, F Zheng Continuous venovenous hemofiltration decreases mortality and ameliorates acute lung injury in canine model of severe salt water drowning Scand J Trauma Resusc Emerg Med, 2016-03-31;24(1):40. 2016-03-31 [PMID: 27036317] (Canine)

FAQs for CXCL8/IL-8 (CA8000). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CXCL8