CaM Kinase I Antibody (S06-7C4)

Western Blot: CaM Kinase I Antibody (S06-7C4) [NBP3-19540] - Western blot detection of CaM Kinase I in Rat Brain, C6, 3T3 cell lysates using NBP3-19540 (1:1000 diluted). Predicted band size: 41kDa. Observed band size: 41kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S06-7C4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

CaM Kinase I Antibody (S06-7C4) Summary

• Description: Novus Biologicals Rabbit CaM Kinase I Antibody (S06-7C4) (NBP3-19540) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human CaM Kinase I (Uniprot # Q14012)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CAMK1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/2000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for CaM Kinase I Antibody (S06-7C4)

• calcium/calmodulin-dependent protein kinase I
• calcium/calmodulin-dependent protein kinase type 1
• CaM kinase I alpha
• CaM Kinase I
• CAMKI
• CaM-KI
• caMKI-alpha
• EC 2.7.11
• EC 2.7.11.17
• MGC120317
• MGC120318

Background

Ca(+2)/calmodulin-dependent protein kinases (CaMKs) are activated upon binding of Ca(+2)/calmodulin. To gain maximal activity, CaMK I and CaMK IV can be further phosphorylated by an upstream kinase, CaMK kinase (CaMKK) (1). CaMKI is a Ca2+/calmodulin-dependent protein kinase that is widely expressed in eukaryotic cells and tissues but for which few, if any, physiological substrates are known (2). CaMKI has been shown to comprise a family of four structurally related isoforms (alpha, beta, gamma, and delta) encoded by separate genes with abundant expression in mature brain. In mouse brain, the four isoforms of CaMKI were found to exhibit distinct spatiotemporal expression during neuronal development. CaMKI may also have possible functional involvement of in the basal dendritic growth of hippocampal neurons (3)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAMK1