CaMKK alpha Antibody

Western Blot: CaMKK alpha Antibody [NBP1-42683] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CaMKK alpha antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CaMKK alpha was also immunoprecipitated by rabbit anti-CaMKK alpha antibody NBP1-42682, which recognizes an upstream epitope.

Immunohistochemistry-Paraffin: CaMKK alpha Antibody [NBP1-42683] - Section of human lung carcinoma (left) and human prostate carcinoma (right). Antibody: Affinity purified rabbit anti- CaMKK alpha used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CaMKK alpha Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 455 and 505 of human calcium/calmodulin-dependent protein kinase kinase 1, alpha using the numbering given in entry NP_757343.1 (GeneID 84254).
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAMKK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100- 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CaMKK alpha Antibody

• calcium/calmodulin-dependent protein kinase kinase 1
• calcium/calmodulin-dependent protein kinase kinase 1, alpha
• Calcium/calmodulin-dependent protein kinase kinase alpha
• CaM-kinase IV kinase
• CaM-kinase kinase 1
• CaM-kinase kinase alpha
• CaM-KK 1
• CAMKK alpha protein
• CaMKK alpha
• CaM-KK alpha
• CaMKK1
• CAMKKACaMKK 1
• DKFZp761M0423
• EC 2.7.11
• EC 2.7.11.17
• MGC34095

Background

The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CaMKK alpha Antibody (NBP1-42683)(2)

Publications using NBP1-42683

• Gan W, Dai X, Dai X et al. LATS suppresses mTORC1 activity to directly coordinate Hippo and mTORC1 pathways in growth control Nat. Cell Biol. 2020-02-01 [PMID: 32015438] (WB, Human)
• Hamada T, Souda M, Yoshimura T et al. Anti-apoptotic Effects of PCP4/PEP19 in Human Breast Cancer Cell Lines: A Novel Oncotarget. Oncotarget. 2014-08-15 [PMID: 25153723]

Bioinformatics

• Gene Symbol: CAMKK1
• Entrez:
  • 84254(Human)