Calretinin Overexpression Lysate (Native)

Images

 
Western Blot: Calretinin Overexpression Lysate (Adult Normal) [NBL1-08641] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Calretinin.

Product Details

Summary
Applications WB

Order Details

Calretinin Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201924 and based on accession number NM_001740. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens calbindin 2 (CALB2), transcript variant CALB2, mRNA.
Gene
CALB2

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
31.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for Calretinin

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Calretinin Overexpression Lysate (Native)

  • 29 kDa calbindin
  • CAB29
  • CAL2
  • CALB2
  • calbindin 2
  • calbindin 2, (29kD, calretinin)
  • calbindin 2, 29kDa (calretinin)
  • calbindin D29K
  • Calretinin
  • CR

Background

This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. Three alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longest protein (isoform 1) which has also been designated as the full-length protein isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Calretinin Lysate (NBL1-08641) (0)

There are no publications for Calretinin Lysate (NBL1-08641).
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Product General Protocols

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Video Protocols

WB Video Protocol

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol CALB2
Entrez

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