Calretinin Antibody - Azide and BSA Free

Images

 
Western Blot: Calretinin Antibody [NBP1-30050] - Rat cerebellum (CB) lysate showing specific immunolabeling of the ~ 29k calretinin protein.
Immunohistochemistry: Calretinin Antibody [NBP1-30050] - Immunostaining of rat cerebellum showing fluorescence of calretinin containing cells.

Product Details

Summary
Reactivity Rt, Hu, Mu, PmSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

Calretinin Antibody - Azide and BSA Free Summary

Description
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
Immunogen
Purified guinea pig calretinin. Accession # P47728
Marker
Mesothelial Cell Marker
Specificity
Specific for the ~29 kDa Calretinin protein in Western blots. The antibody also works well for immunohistochemistry on formalin or paraformaldehyde perfused rat brain tissue sections.
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CALB2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:1000
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Tested in Rat. It is expected that this antibody will also react with human, mouse and non-human primates.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM Tris and 0.1M NaCl
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for Calretinin Antibody - Azide and BSA Free

  • 29 kDa calbindin
  • CAB29
  • CAL2
  • CALB2
  • calbindin 2
  • calbindin 2, (29kD, calretinin)
  • calbindin 2, 29kDa (calretinin)
  • calbindin D29K
  • Calretinin
  • CR

Background

Calbindin 2 (calretinin), closely related to calbindin 1, is an intracellular calcium-binding protein belonging to the troponin C superfamily. Calbindin 1 is known to be involved in the vitamin-D-dependent calcium absorption through intestinal and renal epithelia, while the function of neuronal calbindin 1 and calbindin 2 is poorly understood. The sequence of the calbindin 2 cDNA reveals an open reading frame of 271 codons coding for a protein of 31,520 Da, and shares 58% identical residues with human calbindin 1. Calbindin 2 contains five presumably active and one presumably inactive calcium-binding domains. Comparison with the partial sequences available for chick and guinea pig calbindin 2 reveals that the protein is highly conserved in evolution. The calbindin 2 message was detected in the brain, while absent from heart muscle, kidney, liver, lung, spleen, stomach and thyroid gland. There are two additional forms of alternatively spliced calbindin 2 mRNAs encoding C-terminally truncated proteins. Exon 7 can splice to exon 9, resulting in a frame shift and a translational stop at the second codon of exon 9, and encoding calretinin-20k. Exon 7 can also splice to exon 10, resulting in a frame shift and a translational stop at codon 15 of exon 10, and encoding calretinin-22k. The truncated proteins are able to bind calcium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CALB2
Uniprot