Calretinin Antibody

Western Blot: Calretinin Antibody [NBP1-30050] - Rat cerebellum (CB) lysate showing specific immunolabeling of the ~ 29k calretinin protein.

Immunohistochemistry: Calretinin Antibody [NBP1-30050] - Immunostaining of rat cerebellum showing fluorescence of calretinin containing cells.

• Reactivity: Rt, GP
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated

Calretinin Antibody Summary

• Immunogen: Purified guinea pig calretinin
• Marker: Mesothelial Cell Marker
• Specificity: Specific for the ~29 kDa Calretinin protein in Western blots. The antibody also works well for immunohistochemistry on formalin or paraformaldehyde perfused rat brain tissue sections.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CALB2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunofluorescence 1:500
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Tested in Rat. It is expected that this antibody will also react with human, mouse and non-human primates.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM Tris and 0.1M NaCl
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Calretinin Antibody

• 29 kDa calbindin
• CAB29
• CAL2
• CALB2
• calbindin 2
• calbindin 2, (29kD, calretinin)
• calbindin 2, 29kDa (calretinin)
• calbindin D29K
• Calretinin
• CR

Background

Calbindin 2 (calretinin), closely related to calbindin 1, is an intracellular calcium-binding protein belonging to the troponin C superfamily. Calbindin 1 is known to be involved in the vitamin-D-dependent calcium absorption through intestinal and renal epithelia, while the function of neuronal calbindin 1 and calbindin 2 is poorly understood. The sequence of the calbindin 2 cDNA reveals an open reading frame of 271 codons coding for a protein of 31,520 Da, and shares 58% identical residues with human calbindin 1. Calbindin 2 contains five presumably active and one presumably inactive calcium-binding domains. Comparison with the partial sequences available for chick and guinea pig calbindin 2 reveals that the protein is highly conserved in evolution. The calbindin 2 message was detected in the brain, while absent from heart muscle, kidney, liver, lung, spleen, stomach and thyroid gland. There are two additional forms of alternatively spliced calbindin 2 mRNAs encoding C-terminally truncated proteins. Exon 7 can splice to exon 9, resulting in a frame shift and a translational stop at the second codon of exon 9, and encoding calretinin-20k. Exon 7 can also splice to exon 10, resulting in a frame shift and a translational stop at codon 15 of exon 10, and encoding calretinin-22k. The truncated proteins are able to bind calcium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALB2
• Entrez:
  • 794(Human)
  • 12308(Mouse)
  • 117059(Rat)
• Uniprot:
  • P47728(Rat)