Calretinin Antibody (6A9) - Azide and BSA Free

Western Blot: Calretinin Antibody [NBP3-05530] - Western blot analysis of tissue lysates probed with Calretinin Antibody at a at a dilution of of 1:2,000, in red: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. The single clean band at 29kDa corresponds to the calretinin protein.

Immunocytochemistry/Immunofluorescence: Calretinin Antibody [NBP3-05530] - Immunofluorescent analysis of a section of rat hippocampus section stained with Calretinin Antibody at a at a dilution of of 1:2,000 in green and costained with chicken pAb to parvalbumin at a at a dilution of of 1:1,000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The calretinin and parvalbumin antibodies stain different subsets of GABAergic interneurons.

• Reactivity: Hu, Mu, Rt, Po, Bv, Eq
• Applications: WB, ICC/IF, IHC
• Clone: 6A9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.

Calretinin Antibody (6A9) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Calretinin Antibody (6A9) - Azide and BSA Free (NBP3-05530) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length recombinant human Calretinin expressed in and purified from E. coli.
• Isotype: IgA
• Clonality: Monoclonal
• Host: Mouse
• Gene: CALB2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:2000-5000
  • Immunohistochemistry 1:2000-5000
  • Western Blot 1:2000-5000
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol
• Preservative: No Preservative
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.
• Purity: Unpurified

Alternate Names for Calretinin Antibody (6A9) - Azide and BSA Free

• 29 kDa calbindin
• CAB29
• CAL2
• CALB2
• calbindin 2
• calbindin 2, (29kD, calretinin)
• calbindin 2, 29kDa (calretinin)
• calbindin D29K
• Calretinin
• CR

Background

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALB2