Calponin 1 Antibody (836701) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 836701
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

Calponin 1 Antibody (836701) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human Calponin 1
  Ser2-Ala297
  Accession # P51911
• Specificity: Detects human Calponin 1 in ELISAs. Detects human, mouse and rat Calponin 1 in Western Blots. In direct ELISAs, no cross-reactivity with recombinant human Calponin 3 is observed.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calponin 1 Antibody (836701) [Alexa Fluor® 405]

• Basic calponin
• Calponin 1
• calponin 1, basic, smooth muscle
• Calponin H1
• Calponin H1, smooth muscle
• calponins, basic
• CNN1
• Sm-Calp
• SMCC
• SMCCcalponin-1

Background

CNN-1 (Calponin 1 [calcium and calmodulin-binding troponin T-like protein]; also Calponin basic, CaP and Calponin H1) is a 32-36 kDa cytoplasmic member of the calponin family of proteins. Although reportedly expressed in fibroblasts and endothelial cells, it actually appears to be restricted to smooth muscle and smooth muscle-like cells such as myoepithelium and myofibroblasts in the adult. CNN-1 interacts with F-actin in a phosphorylation-dependent manner. When nonphosphorylated, CNN-1 blocks actomyosin ATPase activity, contributing to the stabilization of actin stress fiber bundles. Thus, CNN-1 expression inhibits cell motility and the formation of podosomes. Human CNN-1 is 297 amino acids (aa) in length. It contains one CH/calponin homology domain (aa 30-127), and three consecutive calponin-like repeats (aa 164-268). The repeats are suggested to mediate actin binding. There are five potential Ser/Thr phosphorylation sites. Full-length human CNN-1 shares 97% aa sequence identity with mouse CNN-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.