Calponin 1 Antibody (836701) [DyLight 488]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 836701
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 488

Calponin 1 Antibody (836701) [DyLight 488] Summary

• Immunogen: E. coli-derived recombinant human Calponin 1
  Ser2-Ala297
  Accession # P51911
• Specificity: Detects human Calponin 1 in ELISAs. Detects human, mouse and rat Calponin 1 in Western Blots. In direct ELISAs, no cross-reactivity with recombinant human Calponin 3 is observed.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: CNN1
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Calponin 1 Antibody (836701) [DyLight 488]

• Basic calponin
• Calponin 1
• calponin 1, basic, smooth muscle
• Calponin H1
• Calponin H1, smooth muscle
• calponins, basic
• CNN1
• Sm-Calp
• SMCC
• SMCCcalponin-1

Background

Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction (1). Calponin binds filamentous actin (F-actin) through two distinct sites ABS1 and ABS2, with an affinity in the low micromolar range (2) Immunoreactivity for calponin, along with alpha-smooth muscle actin and smooth muscle myosin heavy chains, confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adenocarcinomas (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CNN1