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Caldesmon/CALD1 Antibody (CALD1/1424R)

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Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (CALD1/1424R) [NBP2-53225] - Rat Uterus stained with Caldesmon Rabbit Monoclonal Antibody
Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (CALD1/1424R) [NBP2-53225] - Rat Uterus stained with Caldesmon Rabbit Monoclonal Antibody
Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (CALD1/1424R) [NBP2-53225] - Uterus stained with Caldesmon Rabbit Monoclonal Antibody

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P
Clone
CALD1/1424R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-54311AF350
Alexa Fluor 405
NBP2-54311AF405
Alexa Fluor 488
NBP2-54311AF488
Alexa Fluor 532
NBP2-54311AF532
Alexa Fluor 594
NBP2-54311AF594
Alexa Fluor 647
NBP2-54311AF647
Alexa Fluor 700
NBP2-54311AF700
Alexa Fluor 750
NBP2-54311AF750
Allophycocyanin
NBP2-54311APC
Biotin
NBP2-54311B
DyLight 350
NBP2-54311UV
DyLight 405
NBP2-54311V
DyLight 488
NBP2-54311G
DyLight 550
NBP2-54311R
DyLight 594
NBP2-54311DL594
DyLight 650
NBP2-54311C
DyLight 680
NBP2-54311FR
DyLight 755
NBP2-54311IR
FITC
NBP2-54311F
HRP
NBP2-54311H
Janelia Fluor 549
NBP2-54311JF549
Janelia Fluor 646
NBP2-54311JF646
PE
NBP2-54311PE
PerCP
NBP2-54311PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-54311-100ug
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Caldesmon/CALD1 Antibody (CALD1/1424R) Summary

Immunogen
Recombinant human full-length CALD1 protein
Localization
Cytoplasmic
Marker
Smooth Muscle Marker
Protein/Peptide Type
Recombinant Protein
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
CALD1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
Application Notes
Human chromosome location: 7q33
IHC-P:Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer( pH 7.5-8.5) for 10-20 min followed by cooling at RT for 20 minutes
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Caldesmon/CALD1 Antibody (CALD1/1424R)

  • CAD
  • CALD1
  • caldesmon 1
  • Caldesmon
  • CDM
  • CDMH-CAD
  • HCAD
  • LCAD
  • L-CAD
  • MGC21352
  • NAG22

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Calmodulin 2 Antibody [NBP2-14871] - Sample (50 ug of whole cell lysate) A: Mouse brain 15% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: Calmodulin 2 Antibody [NBP2-14871] - DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calmodulin 2 stained by Calmodulin 2 antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.
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Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat testis tissue.
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Calmodulin 3
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Western Blot: Calmodulin 3 Antibody [NBP1-98474] - Titration: 1.0 ug/ml Positive Control: Hela Whole Cell.
Western Blot: Calmodulin 3 Antibody [NBP1-98474] - Human 721_B, Antibody Dilution: 1.0 ug/ml CALM3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.
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Myosin Heavy Chain
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Myosin Heavy Chain was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Myosin Heavy Chain was detected in perfusion fixed frozen sections of mouse skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
(1 Review)
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Desmin Antibody
AF3844
Desmin was detected in immersion fixed frozen sections of mouse skeletal muscle using Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to z-lines. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Desmin was detected in immersion fixed paraffin-embedded sections of human heart using 15 µg/mL Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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ERK2 Antibody
(SZ25-01)
NBP2-67360
Western Blot: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ERK1 Antibody (1E5)
NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Myosin light chain
kinase Antibody (SU40 ...
NBP2-67486
Western Blot: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of MYLK on human lung lysates using anti-MYLK antibody at 1/5,000 dilution.
Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Vimentin Antibody
NB300-223
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Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Human A7 cells. Cells were fixed with 10% formalin and permeabilized with 0.1% TritonX-100. After blocking, cells were incubated with primary antibody (1:600) at 4C O/N followed with Alexa Fluor 488 labeled secondary antibody and counterstained with DAPI and Phalloidin for nuclei and F-actin respectively. Imaged with Leica TCS SP5 confocal microscope. Image from verified customer review.
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CD34 Antibody (MEC
14.7)
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Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.
Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).
(19 Publications)
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MYLK3 Antibody
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Immunohistochemistry-Paraffin: MYLK3 Antibody [NBP2-62682] - Staining of human heart muscle shows high expression.
Immunohistochemistry-Paraffin: MYLK3 Antibody [NBP2-62682] - Analysis in human heart muscle and pancreas tissues using Anti-MYLK3 antibody. Corresponding MYLK3 RNA-seq data are presented for the same tissues.
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Neurogranin
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Immunohistochemistry-Paraffin: Neurogranin Antibody - BSA Free [NBP1-45219] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Vinculin Antibody
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Immunocytochemistry/Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - Mouse primary keratinocytes stained with the clone hVIN-1. Image is courtesy of customer review.
Immunocytochemistry/Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - MCF7 breast carcinoma cells expressing GFP fused CIN85 were labeled at a 1:400 dilution.
Immunocytochemistry/Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - Primary human osteoblast cells labeled (red), actin was stained with Phalloidin (green) and nuclei stained blue.
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PRRT2 Antibody
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Immunohistochemistry-Paraffin: PRRT2 Antibody [NBP1-84168] - Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry-Paraffin: PRRT2 Antibody [NBP1-84168] - Immunohistochemical staining of human cerebellum shows strong positivity in neuropil.
Immunohistochemistry-Paraffin: PRRT2 Antibody [NBP1-84168] - Immunohistochemical staining of human pancreas shows no positivity as expected.
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Rpn2 Antibody
NBP2-38323
Western Blot: Rpn2 Antibody [NBP2-38323] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: Rpn2 Antibody [NBP2-38323] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & actin filaments.
Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human liver.
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MYLK2 Antibody
(2G1)
H00085366-M01
Western Blot: MYLK2 Antibody (2G1) [H00085366-M01] - The antibody was tested in mouse skeletal muscle and shows a band below 75 kDa. This image was submitted via customer Review. *{Mouse WB}*
Western Blot: MYLK2 Antibody (2G1) [H00085366-M01] - Analysis of MYLK2 over-expressed 293 cell line, cotransfected with MYLK2 Validated Chimera RNAi ( Cat # H00085366-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MYLK2 monoclonal antibody (M01), clone 2G1 (Cat # H00085366-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: MYLK2 Antibody (2G1) [H00085366-M01] - Analysis of MYLK2 expression in transfected 293T cell line by MYLK2 monoclonal antibody (M01), clone 2G1.Lane 1: MYLK2 transfected lysate(64.7 KDa).Lane 2: Non-transfected lysate.
(1 Review)
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Caldesmon/CALD1
Antibody (CALD1/1424R
NBP2-53225
Caldesmon/CALD1 Antibody (CALD1/1424R)
Species: Hu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Caldesmon/CALD1 Antibody (NBP2-53225) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-54311AF350
Alexa Fluor 405 NBP2-54311AF405
Alexa Fluor 488 NBP2-54311AF488
Alexa Fluor 532 NBP2-54311AF532
Alexa Fluor 594 NBP2-54311AF594
Alexa Fluor 647 NBP2-54311AF647
Alexa Fluor 700 NBP2-54311AF700
Alexa Fluor 750 NBP2-54311AF750
Allophycocyanin NBP2-54311APC
Biotin NBP2-54311B
DyLight 350 NBP2-54311UV
DyLight 405 NBP2-54311V
DyLight 488 NBP2-54311G
DyLight 550 NBP2-54311R
DyLight 594 NBP2-54311DL594
DyLight 650 NBP2-54311C
DyLight 680 NBP2-54311FR
DyLight 755 NBP2-54311IR
FITC NBP2-54311F
HRP NBP2-54311H
Janelia Fluor 549 NBP2-54311JF549
Janelia Fluor 646 NBP2-54311JF646
PE NBP2-54311PE
PerCP NBP2-54311PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol CALD1