Calbindin D-28K Antibody (CL-300)

Product Discontinued

Calbindin D-28K Antibody (CL-300) Summary

• Immunogen: Purified Calbindin-D from chicken gut.
• Localization: Cytoplasmic, Cell membrane
• Specificity: This is specific to a protein of 28 kDa. Calbindin-D occurs only in a subset of neurons and in a few other tissues. This reacts specifically with Calbindin-D in brain and kidney tissue.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CALB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1/15 - 1/30
• Application Notes: Immunohistochemistry: use for 30 mins at RT (ABC method). Optimal dilution should be determined by the end user. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH 6.0 before commencing with the IHC staining protocol.
• Control:

  Calbindin D-28K Overexpression Lysate

Reactivity Notes

Cross-reacts with Human, Chicken, Fish, Guinea pig, Monkey, Mouse, Rabbit, Rat and Sheep. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: No buffer
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Calbindin D-28K Antibody (CL-300)

• CAB27
• CALB
• calbindin 1, (28kD)
• calbindin 1, 28kDa
• Calbindin D28
• calbindin
• D-28K
• RTVL-H protein
• Vitamin D-dependent calcium-binding protein, avian-type

Background

Calbindin D28k is a member of a large family of intracellular calcium-binding proteins, containing EF-hand calcium binding motifs, and is related to calmodulin and troponin-C. The biological roles of Calbindin D28k include calcium regulation and calcium-dependent signalling in neurons and during development. Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. Calbindin antibody is often used as a marker for cerebellum Purkinje cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Calbindin D-28K Antibody (NB600-561). (Showing 1 - 1 of 1 FAQ).

1. I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?
   • This link will take you to all of our alpha7 nicotinic cholingergic receptor antibodies currently available for purchase. Please see the following link for our Calbinin D28K antibodies reactive against mouse and human. We currently do not have any Parvalbumin antibodies that have been tested in mouse. I would like to introduce you to our Innovators Reward Program. Try our primary antibodies in an untested species or application, without the financial risk of failure. The pricing and availability of our products depends on your country.

Bioinformatics

• Gene Symbol: CALB1
• Entrez:
  • 793(Human)
  • 83839(Rat)