Cadherin-16 Antibody (rCDH16/1071) - Azide and BSA Free

Western Blot: Cadherin-16 Antibody (rCDH16/1071) - Azide and BSA Free [NBP3-08881] - Western Blot Analysis of human kidney tissue lysate using Cadherin-16 Recombinant Mouse Monoclonal Antibody (rCDH16/1071).

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (rCDH16/1071) - Azide and BSA Free [NBP3-08881] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Cadherin-16 Recombinant Mouse Monoclonal Antibody (rCDH16/1071).

Flow Cytometry: Cadherin-16 Antibody (rCDH16/1071) - Azide and BSA Free [NBP3-08881] - Flow Cytometric Analysis of Human HEK293 cells using Cadherin-16 antibody (rCDH16/1071) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

• Reactivity: Hu, Mu, Rt, Ca, Rb
• Applications: WB, Flow, IHC
• Clone: rCDH16/1071
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Cadherin-16 Antibody (rCDH16/1071) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of recombinant monoclonal antibody purified by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07139).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant full-length human Cadherin-16 protein (Uniprot: O75309)
• Localization: Cell Surface and Cytoplasmic
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH16
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cadherin-16 Antibody (rCDH16/1071) - Azide and BSA Free

• cadherin 16, KSP-cadherin
• cadherin-16
• Kidney-specific cadherin
• KSP-cadherin

Background

Cadherin 16 is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDH16