Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Crystallin AB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; ...read more
Immunocytochemistry/ Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Crystallin AB.
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of paraffin-embedded Human pancreas tissue using anti-Crystallin AB mouse monoclonal antibody.
Flow Cytometry: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Crystallin AB ...read more
Immunocytochemistry/ Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of HT29 cells using anti-Crystallin AB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of paraffin-embedded Human endometrium tissue using anti-Crystallin AB mouse monoclonal antibody.
Immunoprecipitation: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 ...read more
20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP1-47708 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for AlphaB Crystallin/CRYAB Antibody (OTI6A9)
alpha B crystallin
alpha-crystallin B chain
CRYA2alpha crystallin B chain
crystallin, alpha B
Heat shock protein beta-5
heat-shock 20 kD like-protein
Renal carcinoma antigen NY-REN-27
Rosenthal fiber component
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development,these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates.Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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