Cadherin-13 Antibody (OTI3H6)

Western Blot: Cadherin-13 Antibody (OTI3H6) [NBP2-02528] - Analysis of extracts (10ug) from 10 Human tissue by using anti-CDH13 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Immunocytochemistry/Immunofluorescence: Cadherin-13 Antibody (OTI3H6) [NBP2-02528] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cadherin-13 Antibody (3H6)

Western Blot: Cadherin-13 Antibody (3H6) [NBP2-02528] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cadherin-13 Antibody (3H6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti- Cadherin-13 Antibody (3H6)

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF
• Clone: OTI3H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.41 mg/ml

Cadherin-13 Antibody (OTI3H6) Summary

• Description: Novus Biologicals Mouse Cadherin-13 Antibody (OTI3H6) (NBP2-02528) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human CDH13 (NP_001248) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH13
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 75.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.41 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cadherin-13 Antibody (OTI3H6)

• cadherin 13, H-cadherin (heart)
• Cadherin13
• Cadherin-13
• CDH13
• CDHHT-cadherin
• H-Cadherin
• Heart cadherin
• heart-cadherin
• P105
• T-cad
• T-Cadherin
• Truncated cadherin
• truncated-cadherin

Background

This gene is a member of the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region but, unlike the typical cadherin superfamily member, lacks the highly conserved cytoplasmic region. This particular cadherin is a putative mediator of cell-cell interaction in the heart and may act as a negative regulator of neural cell growth. The gene locus is hypermethylated or deleted in breast, ovarian and lung cancers. Two major mRNA transcripts encoding identical proteins are found, products of alternative polyadenylation sites

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDH13
• Entrez:
  • 1012(Human)