CA125/MUC16 Antibody (X52) - BSA Free

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: X52
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

CA125/MUC16 Antibody (X52) - BSA Free Summary

• Description: Novus Biologicals Mouse CA125/MUC16 Antibody (X52) - BSA Free (NBP2-11624) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with CA125 antigen purified from human ovarian carcinoma (MW > 1 MDa).
• Epitope: B2 Epitope group
• Specificity: CA125 antigen. Epitope specificity group B (ISOBM class Immunofluorescenceication) similar to M-11
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUC16
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Sandwich ELISA 1:100-1:2000
  • Western Blot
• Application Notes: Detection of CA125 antigen. Suggested pairs for sandwich immunoassay (capture-detection): NB120-10032 - NBP2-11624.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.05% Sodium Azide
• Purity: Ion exchange chromatography

Alternate Names for CA125/MUC16 Antibody (X52) - BSA Free

• CA125 ovarian cancer antigen
• CA125
• CA-125
• CA125MUC-16
• FLJ14303
• MUC16
• mucin 16, cell surface associated
• mucin-16
• Ovarian cancer-related tumor marker CA125
• Ovarian carcinoma antigen CA125

Background

MUC16 (CA125) is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is over expressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller "subunits" being described by some investigators.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CA125/MUC16 Antibody (NBP2-11624). (Showing 1 - 1 of 1 FAQ).

1. We are setting up an ELISA type of assay for CA125. On your web page: Applications: Uses: Detection of CA125 antigen. Suggested pairs for sandwich immunoassay (capture-detection): NB120-10032 (capture) with NBP2-11624 or NB600-1468 as detection. Can you send me more details how these antibodies have been working in your sandwich immunoassay?
   • These antibodies have been tested with very general sandwich ELISA, without any assay optimization, just to determine which MAbs work together as pairs. Coat plate with NB120-10032 in alkaline carbonate buffer Post-coat with casein Add HRP-conjugated NBP2-11624 and antigen to plate Incubate microplates for 45 min at room temperature with shaking Wash 5 times with saline-Tween Add 100 ul of substrate Stop enzymatic reaction addition of 5% sulphuric acid. Measure the optical density. The sensitivity without optimization has been around 5 IU/ml. But we really do not have any recommended protocol and the suitability and sensitivity of the Abs should be determined in your own assay.

Bioinformatics

• Gene Symbol: MUC16
• Uniprot:
  • Q8WXI7()