| Reactivity | HuSpecies Glossary |
| Applications | IHC, IHC-P |
| Clone | C1QB/2961 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | HRP |
| Immunogen | Recombinant fragment (around aa 41-188) of human C1QB protein (exact sequence is proprietary) |
| Localization | Cytoplasmic, Cell Surface |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | C1QB |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A or G purified |
![C1QB Antibody (C1QB/2961) [HRP]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "C1QB Antibody (C1QB/2961) [HRP]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-79920AF350 | |
| Alexa Fluor 405 | NBP2-79920AF405 | |
| Alexa Fluor 488 | NBP2-79920AF488 | |
| Alexa Fluor 532 | NBP2-79920AF532 | |
| Alexa Fluor 594 | NBP2-79920AF594 | |
| Alexa Fluor 647 | NBP2-79920AF647 | |
| Alexa Fluor 700 | NBP2-79920AF700 | |
| Alexa Fluor 750 | NBP2-79920AF750 | |
| Biotin | NBP2-79920B | |
| DyLight 350 | NBP2-79920UV | |
| DyLight 405 | NBP2-79920V | |
| DyLight 488 | NBP2-79920G | |
| DyLight 550 | NBP2-79920R | |
| DyLight 594 | NBP2-79920DL594 | |
| DyLight 650 | NBP2-79920C | |
| DyLight 680 | NBP2-79920FR | |
| DyLight 755 | NBP2-79920IR | |
| FITC | NBP2-79920F | |
| HRP | NBP2-79920H | |
| Janelia Fluor 549 | NBP2-79920JF549 | |
| Janelia Fluor 646 | NBP2-79920JF646 |
Diseases for C1QB Antibody (NBP2-79920H)Discover more about diseases related to C1QB Antibody (NBP2-79920H).
| Pathways for C1QB Antibody (NBP2-79920H)View related products by pathway.
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PTMs for C1QB Antibody (NBP2-79920H)Learn more about PTMs related to C1QB Antibody (NBP2-79920H).
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Identifying tumoral and stromal transcriptomes that underlie tumor plasticity and stromal neuroinflammatory response in brain metastasis By Jamshed Arslan, Pharm. D., PhD. Cancers in the brain often come from tumors elsewhere in the body. Several adaptive mechanisms influence brain metastasis, such as blood brain barrier leakage that can be induced by... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | C1QB |
![Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/images2/Complement-C3-Antibody-(11H9)-Flow-(Intracellular)-NB200-540-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.](http://images.novusbio.com/images2/Complement-C3-Antibody-(11H9)-Immunocytochemistry-Immunofluorescence-NB200-540-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.](http://images.novusbio.com/images2/Complement-C3-Antibody-(11H9)-Immunohistochemistry-Paraffin-NB200-540-img0004.jpg)
![Western Blot: C1q Antibody [NBP1-87492] - Analysis in control (vector only transfected HEK293T lysate) and C1QA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/C1q-Antibody-Western-Blot-NBP1-87492-img0012.jpg)
![Immunohistochemistry-Paraffin: C1q Antibody [NBP1-87492] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/images2/C1q-Antibody-Immunohistochemistry-Paraffin-NBP1-87492-img0010.jpg)
![Western Blot: C1q Antibody [NBP1-87492] - Analysis in human spleen tissue.](http://images.novusbio.com/images2/C1q-Antibody-Western-Blot-NBP1-87492-img0006.jpg)
![Western Blot: Complement Component C1qC Antibody (4H9D7) [NBP2-61867] - Analysis using C1QC mAb against human C1QC (AA: 115-245) recombinant protein. (Expected MW is 40 kDa)](http://images.novusbio.com/images2/Complement-Component-C1qC-Antibody-(4H9D7)-Western-Blot-NBP2-61867-img0003.jpg)
![Flow Cytometry: Complement Component C1qC Antibody (4H9D7) [NBP2-61867] - Analysis of HeLa cells using C1QC mouse mAb (green) and negative control (red).](http://images.novusbio.com/images2/Complement-Component-C1qC-Antibody-(4H9D7)-Flow-Cytometry-NBP2-61867-img0002.jpg)
![Western Blot: Complement Component C1qC Antibody (4H9D7) [NBP2-61867] - Analysis using C1QC mAb against HEK293 (1) and C1QC (AA: 115-245)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/images2/Complement Component C1qC Antibody (4H9D7)-Western Blot-NBP2-61867-img0004.jpg)
![ELISA: Human Complement Component C2 ELISA Kit (Colorimetric) [NBP2-60548] - Standard curve.](http://images.novusbio.com/images2/Human-Complement-Component-C2-ELISA-Kit-(Colorimetric)-ELISA-NBP2-60548-img0001.jpg)
![Western Blot: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(SN0652)-Western-Blot-NBP2-67656-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in B16-F1 (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/hnRNP C1 + C2 Antibody (SN0652)-Immunocytochemistry-Immunofluorescence-NBP2-67656-img0010.jpg)
![Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded mouse skin tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunohistochemistry-Paraffin-NBP2-67656-img0008.jpg)
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![Flow Cytometry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of CD45 (30F11) in freshly isolated mouse (Balb C) splenocytes (1x10^6) were stained with [NB100-77417] at 1:1000 dilution and detected with FITC conjugated donkey anti-rat IgG secondary (orange). Shown with rat IgG isotype control (blue).](http://images.novusbio.com/images2/CD45-Antibody-(30-F11)-Flow-Cytometry-NB100-77417-img0004.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Analysis of FFPE section of mouse spleen using rat monoclonal CD45 antibody (30-F11) [NB100-77417] at 1:50 dilution. The antibody generated an expected membrane staining in the red and white pulp spleenocytes of the tested spleen section.](http://images.novusbio.com/images2/CD45-Antibody-(30-F11)-Immunohistochemistry-Paraffin-NB100-77417-img0006.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Staining of a FFPE mouse liver tissue section using 1:100 dilution of CD45 antibody (30-F11) [NB100-77417]. Signal was detected using AF488 conjugated donkey anti-rat secondary antibody at 1:500 dilution. Image submitted via verified customer review.](http://images.novusbio.com/images2/CD45-Antibody-(30-F11)-Immunohistochemistry-Paraffin-NB100-77417-img0002.jpg)
![Western Blot: PPAR delta/NR1C2 Antibody [NB600-637] - Detection a predominant band at 43 kDa corresponding to PPAR delta present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4 C followed reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDye 800 fluorescence](http://images.novusbio.com/images2/PPAR-delta-NR1C2-Antibody-Western-Blot-NB600-637-img0004.jpg)
![Immunohistochemistry: PPAR delta/NR1C2 Antibody [NB600-637] - Rabbit Anti-PPAR delta (N terminal specific).](http://images.novusbio.com/images2/PPAR-delta-NR1C2-Antibody-Immunohistochemistry-NB600-637-img0003.jpg)
![Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Detection of Human SRC3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC3 antibody NB100-314 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/images2/NCOA3-AIB1-Antibody-Western-Blot-NB100-314-img0004.jpg)
![Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Nuclear extract (5 mcg for WB, 10 mg for CoIP) from HeLa cells. Affinity purified rabbit anti-SRC3 used at 0.33 mcg/ml for WB and 20 mcg/10 mg extract for CoIP.](http://images.novusbio.com/images2/NCOA3-AIB1-Antibody-Western-Blot-NB100-314-img0002.jpg)
![Western Blot: Complement Component C1s Antibody (4E3) [NBP2-01625] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C1s (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Complement Component C1s.](http://images.novusbio.com/images2/Complement-Component-C1s-Antibody-(4E3)-Western-Blot-NBP2-01625-img0025.jpg)
![Immunocytochemistry/Immunofluorescence: Complement Component C1s Antibody (OTI4E3) [NBP2-01625] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY C1s.](http://images.novusbio.com/images2/Complement-Component-C1s-Antibody-(4E3)-Immunocytochemistry-Immunofluorescence-NBP2-01625-img0016.jpg)
![Immunohistochemistry-Paraffin: Complement Component C1s Antibody (OTI4E3) [NBP2-01625] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-C1S mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/images2/Complement-Component-C1s-Antibody-(OTI4E3)-Immunohistochemistry-Paraffin-NBP2-01625-img0027.jpg)
![Knockout Validated: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/HSP27-Antibody-Knockout-Validated-NBP1-75477-img0010.jpg)
![Western Blot: HSP27 Antibody [NBP1-75477] - Analysis of Jurkat cell lysate (30ug) using anti-Hsp27 antibody. Image from verified customer review.](http://images.novusbio.com/images2/Hsp27-Antibody-Western-Blot-NBP1-75477-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP1-75477] - Hsp27 Antibody [NBP1-75477] - HSP27 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/images2/Hsp27 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75477-img0006.jpg)