C-Reactive Protein/CRP Antibody (233539) [Alexa Fluor® 750]

• Applications: ELISA(Cap), ELISA(Det)
• Clone: 233539
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

C-Reactive Protein/CRP Antibody (233539) [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat C‑Reactive Protein/CRP
  His20-Ser230
  Accession # P48199
• Specificity: Detects rat C‑Reactive Protein/CRP in direct ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant mouse CRP, recombinant porcine CRP, recombinant human (rh) Pentraxin 2, or rhPentraxin 3 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

CRP is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. The rat CRP gene encodes a 230 amino acid (aa) precursor with a signal peptide of 19 aa and the mature polypeptide of 211 aa. Rat CRP shares 64% and 70% aa sequence homology with human and mouse CRP respectively. Human, mouse and rabbit CRP are non-glycosylated proteins, and the units are non-covalently linked to form the pentameter. In contrast, rat CRP is a glycoprotein and contains a covalently linked dimer in the pentameter. CRP exhibits Ca⁺⁺-dependent binding to ligands. Phosphocholine (PCh), a constituent of many bacterial and fungal walls, is a principal ligand of CRP. CRP also binds to the membrane of injured cells, the membrane and nuclear components of necrotic and apoptotic cells. Upon binding with the ligands, CRP is recognized by C1q and initiates the activation of complement cascade. Ligand bound CRP also binds to Fc gamma RI and Fc gamma RIIa on phagocytes and activates phagocytotic responses. In addition to phagocytosis, CRP also induces the production of hydrogen peroxide and inflammatory cytokines, such as IL-1, IL-6 and TNF-alpha . In human and rabbits, CRP is an important acute-phase protein that plays a role in the first line of host innate defense. The level of plasma CRP at basal conditions in human and rabbits is very low, and can increase 1,000-fold within 24‑48 hours in response to infection, inflammation or tissue damage. In rats, CRP exists at a high level at basal conditions and only increases about 2-fold in response to injury. CRP is not a typical acute-phase protein in rat and is a minor component in response to injury. In mice, CRP is expressed at very low levels and is not an acute phase reactant. Serum amyloid P component (SAP), another pentraxin, is an acute phase serum protein in mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.