c-Myb Antibody

Western Blot: c-Myb Antibody [H00004602-B01P] - Analysis of MYB expression in transfected 293T cell line by MYB polyclonal antibody. Lane 1: MYB transfected lysate(70.4 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: c-Myb Antibody [H00004602-B01P] - Analysis of purified antibody to MYB on HeLa cell. (antibody concentration 10 ug/ml)

Product Discontinued

c-Myb Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: MYB (NP_005366.2, 1 a.a. - 640 a.a.) full-length human protein. MARRPRHSIYSSDEDDEDFEMCDHDYDGLLPKSGKRHLGKTRWTREEDEKLKKLVEQNGTDDWKVIANYLPNRTDVQCQHRWQKVLNPELIKGPWTKEEDQRVIELVQKYGPKRWSVIAKHLKGRIGKQCRERWHNHLNPEVKKTSWTEEEDRIIYQAHKRLGNRWAEIAKLLPGRTDNAIKNHWNSTMRRKVEQEGYLQESSKASQPAVATSFQKNSHLMGFAQAPPTAQLPATGQPTVNNDYSYYHISEAQNVSSHVPYPVALHVNIVNVPQPAAAAIQRHYNDEDPEKEKRIKELELLLMSTENELKGQQVLPTQNHTCSYPGWHSTTIADHTRPHGDSAPVSCLGEHHSTPSLPADPGSLPEESASPARCMIVHQGTILDNVKNLLEFAETLQFIDSFLNTSSNHENSDLEMPSLTSTPLIGHKLTVTTPFHRDQTVKTQKENTVFRTPAIKRSILESSPRTPTPFKHALAAQEIKYGPLKMLPQTPSHLVEDLQDVIKQESDESGIVAEFQENGPPLLKKIKQEVESPTDKSGNFFCSHHWEGDSLNTQLFTQTSPVADAPNILTSSVLMAPASEDEDNVLKAFTVPKNRSLASPLQPCSSTWEPASCGKMEEQMTSSSQARKYVNAFSARTLVM
• Specificity: MYB - v-myb myeloblastosis viral oncogene homolog (avian),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: MYB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for c-Myb Antibody

• c-myb protein (140 AA)
• Cmyb
• c-myb
• c-myb_CDS
• c-myb10A_CDS
• c-myb13A_CDS
• c-myb14A_CDS
• c-myb8B_CDS
• efg
• Proto-oncogene c-Myb
• transcriptional activator Myb
• v-myb avian myeloblastosis viral oncogene homolog
• v-myb myeloblastosis viral oncogene homolog (avian)

Background

The c-Myb proto-oncogene is a 75 kDa protein involved in growth regulation and differentiation in many different cell types but it is predominantly expressed in immature hemopoietic cells where it plays an important role in cell proliferation. c-Myb activity is directly regulated by cyclin D1 and CDKs and it is believed that c-Myb activity is regulated during the cell cycle in hematopoietic cells (2). Disrupting c-myb function might, therefore, prove an effective therapeutic strategy for controlling leukemic cell growth (3). c-Myb binds to promoter sequences of genes such as c-Myc or Bcl-2 that are expressed in cutaneous T-cell lymphoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYB
• Entrez:
  • 4602(Human)
• Uniprot:
  • NP_005366.2(Human)