c-Myb [p Ser11] Antibody (SZ04-81)

Immunocytochemistry/Immunofluorescence: c-Myb [p Ser11] Antibody (SZ04-81) [NBP2-67368] - Staining P-V-Myb+C-Myb(S11) in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: c-Myb [p Ser11] Antibody (SZ04-81) [NBP2-67368] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-c-Myb antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: c-Myb [p Ser11] Antibody (SZ04-81) [NBP2-67368] - Analysis of MCF-7 cells with P-V-Myb+C-Myb(S11) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as antibody

Product Discontinued

c-Myb [p Ser11] Antibody (SZ04-81) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser11 of human V-Myb+C-Myb. (SwissProt: P10242 Human)
• Modification: p Ser11
• Localization: Nucleus, cytosol.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MYB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for c-Myb [p Ser11] Antibody (SZ04-81)

• c-myb protein (140 AA)
• Cmyb
• c-myb
• c-myb_CDS
• c-myb10A_CDS
• c-myb13A_CDS
• c-myb14A_CDS
• c-myb8B_CDS
• efg
• Proto-oncogene c-Myb
• transcriptional activator Myb
• v-myb avian myeloblastosis viral oncogene homolog
• v-myb myeloblastosis viral oncogene homolog (avian)

Background

The c-Myb proto-oncogene is a 75 kDa protein involved in growth regulation and differentiation in many different cell types but it is predominantly expressed in immature hemopoietic cells where it plays an important role in cell proliferation. c-Myb activity is directly regulated by cyclin D1 and CDKs and it is believed that c-Myb activity is regulated during the cell cycle in hematopoietic cells (2). Disrupting c-myb function might, therefore, prove an effective therapeutic strategy for controlling leukemic cell growth (3). c-Myb binds to promoter sequences of genes such as c-Myc or Bcl-2 that are expressed in cutaneous T-cell lymphoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYB