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c-Maf Antibody (883926) [Unconjugated]

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Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. Gels were loaded with 40 μg of cytoplasmic (Cyto) and 20 μg of nuclear (Nuc) extracts. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human c-Maf Monoclonal Antibody (Catalog # MAB8227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for c-Maf at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. Gels were loaded with 40 μg of cytoplasmic (Cyto) and 20 μg of nuclear (Nuc) extracts. PVDF membrane was probed with 2 µg/mL of Mouse ...read more
c-Maf was detected in immersion fixed RPMI 8226 human multiple myeloma cell line using Mouse Anti-Human c-Maf Monoclonal Antibody (Catalog # MAB8227) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
883926
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB8227-SP
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c-Maf Antibody (883926) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human c-Maf
Met1-Phe372
Accession # O75444
Specificity
Detects human c-Maf in direct ELISA and Western Blot.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MAF
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 3
using
MAB8227 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for c-Maf Antibody (883926) [Unconjugated]

  • Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene
  • CCA4
  • c-maf proto-oncogene
  • cMaf
  • c-Maf
  • MAF2
  • MGC71685
  • Proto-oncogene c-Maf
  • T lymphocyte c-maf long form
  • transcription factor Maf
  • v-maf musculoaponeurotic fibrosarcoma (avian) oncogene homolog
  • v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian)
  • V-maf musculoaponeurotic fibrosarcoma oncogene homolog

Background

c-Maf is an approximately 40 kDa transcriptional regulator that contains one bZIP domain. It can associate into homodimers and heterodimers with other basic leucine zipper transcription factors. c-Maf plays an important role in fetal erythropoiesis, lens development, mechanosensory neuron development, and the differentiation of chondrocytes and osteoblasts. In the pancreas, c-Maf promotes the transcription of glucagon in alpha cells and insulin in beta cells. In immune cells, it controls Th17 and Treg differentiation by regulating the transcription of IL-4, IL-12 p35, IL-21, IL-22, and GM-CSF. Human c-Maf shares 97% amino acid sequence identity with mouse and rat c-Maf. Alternative splicing of human c-Maf generates a long isoform with a 30 aa substitution for the C-terminal methionine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for c-Maf Antibody (MAB8227) (0)

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Review for c-Maf Antibody (MAB8227) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB8227:
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ICC/IF
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Images Ratings Applications Species Date Details
Immunocytochemistry/Immunofluorescence c-Maf MAB8227
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reviewed by:
Natalie Erdmann
 ICC/IF Human 10/24/2016
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedRPMI-8226
SpeciesHuman
LotCIHK051508A

Comments

CommentsImmersion-fixed cells; permeabilized Triton X-100 5 min; blocked 1 hour 10% donkey serum; 1/30 O/N @4oC; Donkey F(ab’)2 anti-mouse IgG (H+L) Alexa Fluor 647 Abcam #ab150103 1 hour at rt; Zeiss AxioImager Cy5 filter cube 500 ms exposure

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Immunocytochemistry/Immunofluorescence c-Maf MAB8227
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 Natalie Erdmann
10/24/2016
Application: ICC/IF
Species: Human
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Bioinformatics

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