BXDC5 Antibody

Images

 
Western Blot: BXDC5 Antibody [NBP1-89915] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: BXDC5 Antibody [NBP1-89915] - Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoli.
Immunohistochemistry-Paraffin: BXDC5 Antibody [NBP1-89915] - Staining of human hippocampus shows distinct nucleolar positivity in neuronal cells.
Western Blot: BXDC5 Antibody [NBP1-89915] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Product Details

Summary
Reactivity Hu, Mu, Rt, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

BXDC5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:SGKKSLKRKAAAEELQEAAGAGDGATENGVQPPKAAAFPPGFSISEIKNKQRRHLMFTRWKQQQRKEKLAAKKKLKKEREALGDKAPPKPVPKTID
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:20-1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
BXDC5 Protein (NBP1-89915PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Rat (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for BXDC5 Antibody

  • 2310066N05Rik
  • BBR140
  • brix domain containing 5
  • Brix domain-containing protein 5
  • BXDC5
  • DKFZp761G0415
  • DKFZp761M0215
  • FLJ12475
  • Ribosome biogenesis protein RPF1
  • ribosome production factor 1 homolog (S. cerevisiae)
  • ribosome production factor 1
  • RNA processing factor 1 (RPF1)
  • RP11-118B23.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RPF1