BubR1 Antibody

ELISA: BubR1 Antibody [23730002]

Product Discontinued

BubR1 Antibody Summary

• Immunogen: This antibody is specific for the N Terminus Region of the target protein.
• Specificity: This product is specific for Human BUB1B.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BUB1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA and Western Blot.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for BubR1 Antibody

• Bub1A
• BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta
• budding uninhibited by benzimidazoles 1 homolog beta (yeast)
• EC 2.7.11.1
• hBUBR1
• MAD3/BUB1-related protein kinase
• MAD3LBUB1beta
• Mitotic checkpoint kinase MAD3L
• Protein SSK1
• SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Background

Compromised spindle checkpoints are thought to play an important role in genetic instability that predisposes cells to malignant transformations. Human cells contain two related checkpoint protein kinases, evolved from the BUB1 gene; hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotic kinetochore-microtubule interactions. hBUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. It has been identified as a novel binding partner of BCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate the progression of breast cancer by compromising the mitotic checkpoint control through inactivation of BUBR1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BubR1 Antibody (23730002)(2)

Publications using 23730002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: BUB1B
• Entrez:
  • 701(Human)
• Uniprot:
  • O60566(Human)