Bub1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HEK293 human embryonic kidney cell line and nuclear extracts of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human Bub1 Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Bub1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Bub1
Met1-Ser250
Accession # O43683
Specificity
Detects human Bub1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BUB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Control
Bub1 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bub1 Antibody [Unconjugated]

  • BUB1 budding uninhibited by benzimidazoles 1 homolog
  • Bub1
  • BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1
  • BUB1L
  • budding uninhibited by benzimidazoles 1 (yeast homolog)
  • budding uninhibited by benzimidazoles 1 homolog (yeast)
  • EC 2.7.11.1
  • hBUB1mitotic spindle checkpoint kinase
  • putative serine/threonine-protein kinase

Background

The Bub1 (Budding uninhibited by benzimidazoles-1) protein is a kinase that acts in the mitotic spindle checkpoint. Bub1 delays chromosome segregation in response to genotoxic stress. Mutations or the absence of Bub1 can lead to aneuploidy and chromosomal instability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BUB1
Uniprot