Bub1 Antibody [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line and nuclear extracts of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human Bub1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3785) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Bub1 was detected at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Bub1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Bub1
  Met1-Ser250
  Accession # O43683
• Specificity: Detects human Bub1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BUB1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bub1 Antibody [Unconjugated]

• BUB1 budding uninhibited by benzimidazoles 1 homolog
• Bub1
• BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1
• BUB1L
• budding uninhibited by benzimidazoles 1 (yeast homolog)
• budding uninhibited by benzimidazoles 1 homolog (yeast)
• EC 2.7.11.1
• hBUB1mitotic spindle checkpoint kinase
• putative serine/threonine-protein kinase

Background

The Bub1 (Budding uninhibited by benzimidazoles-1) protein is a kinase that acts in the mitotic spindle checkpoint. Bub1 delays chromosome segregation in response to genotoxic stress. Mutations or the absence of Bub1 can lead to aneuploidy and chromosomal instability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BUB1
• Uniprot:
  • O43683()