![Western Blot: BTF Antibody (M33-P5B11) [NBP2-50118] - Western Blot using mAb M33-P5B11.](http://images.novusbio.com/fullsize/BTF-Antibody-M33-P5B11-Western-Blot-NBP2-50118-img0005.jpg "Western Blot: BTF Antibody (M33-P5B11) [NBP2-50118] - Western Blot using mAb M33-P5B11.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clone | M33-P5B11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Peptide Sequence - KYQGDGIVED (amino acids 900 - 909) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BCLAF1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Positive control(s): ELISA- Peptide immunogen; Western Blot- Hela whole cell extract; IHC- Human colon carcinoma. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for BTF Antibody (NBP2-50118)Find related products by research area.
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| Gene Symbol | BCLAF1 |
![Immunohistochemistry: BTF Antibody (M33-P5B11) [NBP2-50118] - IHC was performed on formalin-fixed, paraffin-embedded tissue sections using Anti-BCLAF1 [M33-P5B11]. Image is showing both cytoplasmic and nuclear localisation positivity of BCLAF1 in colon cancer which is in agreement with existing literature. Exclusively nuclear localisation of BCLAF1 is observed in non-neoplastic colon mucosa. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).](http://images.novusbio.com/fullsize/BTF-Antibody-M33-P5B11-Immunohistochemistry-NBP2-50118-img0002.jpg "Immunohistochemistry: BTF Antibody (M33-P5B11) [NBP2-50118] - IHC was performed on formalin-fixed, paraffin-embedded tissue sections using Anti-BCLAF1 [M33-P5B11]. Image is showing both cytoplasmic and nuclear localisation positivity of BCLAF1 in colon cancer which is in agreement with existing literature. Exclusively nuclear localisation of BCLAF1 is observed in non-neoplastic colon mucosa. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")