Brk Antibody

Western Blot: Brk Antibody [H00005753-B01P] - Analysis of PTK6 expression in human colon.

Immunocytochemistry/Immunofluorescence: Brk Antibody [H00005753-B01P] - Analysis of purified antibody to PTK6 on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: Brk Antibody [H00005753-B01P] - Analysis of PTK6 expression in transfected 293T cell line by PTK6 polyclonal antibody. Lane 1: PTK6 transfected lysate(49.61 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

Brk Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: PTK6 (NP_005966.1, 1 a.a. - 451 a.a.) full-length human protein. MVSRDQAHLGPKYVGLWDFKSRTDEELSFRAGDVFHVARKEEQWWWATLLDEAGGAVAQGYVPHNYLAERETVESEPWFFGCISRSEAVRRLQAEGNATGAFLIRVSEKPSADYVLSVRDTQAVRHYKIWRRAGGRLHLNEAVSFLSLPELVNYHRAQSLSHGLRLAAPCRKHEPEPLPHWDDWERPREEFTLCRKLGSGYFGEVFEGLWKDRVQVAIKVISRDNLLHQQMLQSEIQAMKKLRHKHILALYAVVSVGDPVYIITELMAKGSLLELLRDSDEKVLPVSELLDIAWQVAEGMCYLESQNYIHRDLAARNILVGENTLCKVGDFGLARLIKEDVYLSHDHNIPYKWTAPEALSRGHYSTKSDVWSFGILLHEMFSRGQVPYPGMSNHEAFLRVDAGYRMPCPLECPPSVHKLMLTCWCRDPEQRPCFKALRERLSSFTSYENPT
• Specificity: PTK6 - PTK6 protein tyrosine kinase 6,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: PTK6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It is also useful for IF.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Brk Antibody

• Breast tumor kinase
• Brk
• BRKprotein-tyrosine kinase 6
• EC 2.7.10
• EC 2.7.10.2
• FLJ42088
• protein-tyrosine kinase BRK
• PTK6 protein tyrosine kinase 6
• PTK6
• Sik
• Tksk
• Tyrosine-protein kinase BRK

Background

The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Brk Antibody (H00005753-B01P)(3)

Publications using H00005753-B01P

• Sallam AA, Mohyeldin MM, Foudah AI et al. Marine natural products-inspired phenylmethylene hydantoins with potent in vitro and in vivo antitumor activities via suppression of Brk and FAK signaling. Org Biomol Chem. 2014-06-13 [PMID: 24927150]
• Akl MR, Foudah AI, Ebrahim HY et al. The marine-derived sipholenol A-4-O-3',4'-dichlorobenzoate inhibits breast cancer growth and motility in vitro and in vivo through the suppression of Brk and FAK signaling. Mar Drugs. 2014-04-14 [PMID: 24736807]
• Foudah AI, Sallam AA, Akl MR, El Sayed KA. Optimization, Pharmacophore Modeling and 3D-QSAR Studies of Sipholanes as Breast Cancer Migration and Proliferation Inhibitors. Eur J Med Chem. 2013-12-12 [PMID: 24487236]

Bioinformatics

• Gene Symbol: PTK6
• Entrez:
  • 5753(Human)
• Uniprot:
  • NP_005966.1(Human)