Brevican Antibody - BSA Free

Western Blot: Brevican Antibody [NBP2-15616] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Brevican antibody [N1N2], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Brevican Antibody [NBP2-15616] - Human breast cancer, using Brevican antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Brevican Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Brevican. The exact sequence is proprietary.
• Localization: Isoform 1: Secreted; extracellular space; extracellular matrix; Isoform 2: Membrane; Lipid-anchor; GPI-anchor
• Predicted Species: Bovine (96%), Canine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCAN
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID:30158698).
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 99 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse and Rat reactivity reported in scientific literature (PMID: 30158698).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Brevican Antibody - BSA Free

• ALPBRE
• BCAN
• BEHAB
• BEHABbrevican core protein
• Brain-enriched hyaluronan-binding protein
• Brevican
• Chondroitin sulfate proteoglycan 7
• chondroitin sulfate proteoglycan BEHAB
• chondroitin sulfate proteoglycan BEHAB/brevican
• CSPG7
• CSPG7brevican proteoglycan
• MGC13038

Background

BCAN - brevican

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Brevican Antibody (NBP2-15616)(2)

Publications using NBP2-15616

• Wu L, Pandey V, Casha V et al. The cell-surface shared proteome of astrocytes and neurons and the molecular foundations of their multicellular interactions. Neuron 2025-06-05 [PMID: 40499536]
• Anderson MA, O'Shea TM, Burda JE et al. Required growth facilitators propel axon regeneration across complete spinal cord injury Nature 2018-08-31 [PMID: 30158698] (ICC/IF, Rat, Mouse)

Bioinformatics

• Gene Symbol: BCAN
• Uniprot:
  • Q96GW7(Human)