![Immunohistochemistry-Paraffin: BRD9 Antibody [NBP2-15614] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/BRD9-Antibody-Immunohistochemistry-Paraffin-NBP2-15614-img0002.jpg "Immunohistochemistry-Paraffin: BRD9 Antibody [NBP2-15614] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.")
| Reactivity | Hu, Mu, Rt, Bv, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Novus Biologicals Rabbit BRD9 Antibody (NBP2-15614) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human BRD9. The exact sequence is proprietary. |
| Predicted Species | Mouse (96%), Rat (97%), Rhesus Macaque (100%), Bovine (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BRD9 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ICC/IF Assay dependent |
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| Theoretical MW | 67 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 1% BSA, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
| Publication using NBP2-15614 | Applications | Species |
|---|---|---|
| Wu Z, Pope S, Ahmed N et al. Regulation of inflammatory responses by pH-dependent transcriptional condensates. Cell 2025-10-03 [PMID: 40680734] |
Secondary Antibodies |
Isotype Controls |
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![ICC/IF-BRD9 Antibody [NBP2-15614] -A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRD9 stained by BRD9 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.](http://images.novusbio.com/fullsize/antibody/nbp2-15614_rabbit-polyclonal-brd9-antibody-immunocytochemistry-immunofluorescence-762023113543..jpg "ICC/IF-BRD9 Antibody [NBP2-15614] -A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRD9 stained by BRD9 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.")
![Western Blot: BRD9 Antibody [NBP2-15614] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BRD9 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-15614_rabbit-polyclonal-brd9-antibody-western-blot-762023113725..jpg "Western Blot: BRD9 Antibody [NBP2-15614] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BRD9 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")