![Immunocytochemistry/Immunofluorescence: BRD9 Antibody (HL1282) - Azide and BSA Free [NBP3-25385] - BRD9 antibody [HL1282] detects BRD9 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRD9 stained by BRD9 antibody [HL1282] (NBP3-25385) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25385_rabbit-brd9-mab-hl1282-azide-and-bsa-free-2120249383127.jpg "Immunocytochemistry/Immunofluorescence: BRD9 Antibody (HL1282) - Azide and BSA Free [NBP3-25385] - BRD9 antibody [HL1282] detects BRD9 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRD9 stained by BRD9 antibody [HL1282] (NBP3-25385) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.")
| Reactivity | Hu, Mu, Rt, Bv, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1282 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Rabbit BRD9 Antibody (HL1282) - Azide and BSA Free (NBP3-25385) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human BRD9. The exact sequence is proprietary. |
| Source | HEK293 |
| Predicted Species | Rhesus Macaque (100%), Bovine (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | BRD9 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BRD9 |
![Immunohistochemistry-Paraffin: BRD9 Antibody (HL1282) - Azide and BSA Free [NBP3-25385] - BRD9 antibody [HL1282] detects BRD9 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. BRD9 stained by BRD9 antibody [HL1282] (NBP3-25385) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25385_rabbit-brd9-mab-hl1282-azide-and-bsa-free-2120249413325.jpg "Immunohistochemistry-Paraffin: BRD9 Antibody (HL1282) - Azide and BSA Free [NBP3-25385] - BRD9 antibody [HL1282] detects BRD9 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. BRD9 stained by BRD9 antibody [HL1282] (NBP3-25385) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: BRD9 Antibody (HL1282) - Azide and BSA Free [NBP3-25385] - BRD9 antibody [HL1282] detects BRD9 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. BRD9 stained by BRD9 antibody [HL1282] (NBP3-25385) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25385_rabbit-brd9-mab-hl1282-azide-and-bsa-free-212024940830.jpg "Immunohistochemistry-Paraffin: BRD9 Antibody (HL1282) - Azide and BSA Free [NBP3-25385] - BRD9 antibody [HL1282] detects BRD9 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. BRD9 stained by BRD9 antibody [HL1282] (NBP3-25385) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: BRD9 Antibody (HL1282) - Azide and BSA Free [NBP3-25385] - BRD9 antibody [HL1282] detects BRD9 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. BRD9 stained by BRD9 antibody [HL1282] (NBP3-25385) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25385_rabbit-brd9-mab-hl1282-azide-and-bsa-free-2120249415910.jpg "Immunohistochemistry-Paraffin: BRD9 Antibody (HL1282) - Azide and BSA Free [NBP3-25385] - BRD9 antibody [HL1282] detects BRD9 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. BRD9 stained by BRD9 antibody [HL1282] (NBP3-25385) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: BRD9 Antibody (HL1282) - Azide and BSA Free [NBP3-25385] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BRD9 antibody [HL1282] (NBP3-25385) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25385_rabbit-brd9-mab-hl1282-azide-and-bsa-free-2120249342735.jpg "Western Blot: BRD9 Antibody (HL1282) - Azide and BSA Free [NBP3-25385] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BRD9 antibody [HL1282] (NBP3-25385) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: BRD9 Antibody (HL1282) - Azide and BSA Free [NBP3-25385] - K562 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BRD9 antibody [HL1282] (NBP3-25385) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25385_rabbit-brd9-mab-hl1282-azide-and-bsa-free-2120249351374.jpg "Western Blot: BRD9 Antibody (HL1282) - Azide and BSA Free [NBP3-25385] - K562 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BRD9 antibody [HL1282] (NBP3-25385) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")