BRD9 Antibody (BLR153J)

Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730] - Whole cell lysate (30 ug) from CT26, CH27, and TCMK-1 cellsprepared using NETN lysis buffer. Antibody: Rabbit antiBRD9 recombinant monoclonal antibody [BLR153J] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes.Lower Panel: Rabbit anti-Actin recombinant monoclonalantibody [BLR057F].

Simple Western: BRD9 Antibody [NBP3-14730] - Detection of human BRD9 by Simple Western. Samples: Whole cell lysate (0.4 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (NBP3-14730) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image internally validated.

Simple Western: BRD9 Antibody [NBP3-14730] - Detection of mouse BRD9 by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (NBP3-14730) used at 1:50 and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image internally validated.

Immunocytochemistry/Immunofluorescence: BRD9 Antibody (BLR153J) [NBP3-14730] - Section of HDLM-2 cells. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J]. (Secondary: HRPconjugated goat anti-rabbit IgG.

Immunohistochemistry-Paraffin: BRD9 Antibody (BLR153J) [NBP3-14730] - Section of mouse plasmacytoma. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J]. Secondary: HRP-conjugatedgoat anti-rabbit IgG.

Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730] - Whole cell lysate (50 ug) prepared using NETN buffer fromJurkat cells (WT and KO). Antibody: Rabbit anti-BRD9recombinant monoclonal antibody [BLR153J] used at 1:1000. Secondary: HRP-conjugated goat antirabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730] - Whole cell lysate (30 ug) from Jurkat, HEK293T, K-562, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.

Immunohistochemistry-Paraffin: BRD9 Antibody (BLR153J) [NBP3-14730] - Section of lung carcinoma. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J]. Secondary: HRP-conjugated goat antirabbit IgG.

Immunoprecipitation: BRD9 Antibody [NBP3-14730] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-BRD9recombinant monoclonal antibody [BLR153J] (A700-153 lot2) used for IP at 20 ul/mg lysate. BRD9 was alsoimmunoprecipitated by a previous lot of this antibodyand a second antibody against a differentepitope of BRD9. For blottingimmunoprecipitated BRD9, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.

Antibody: Rabbit anti-BRD9 recombinantmonoclonal antibody [BLR153J] or isotypecontrol (unshaded). Secondary: DyLight® 650-conjugatedgoat anti-rabbit IgG.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP
• Clone: BLR153J
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

BRD9 Antibody (BLR153J) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: between 547 and 597 (C-term)
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BRD9
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 6 ul/1 mg lysate
  • Simple Western 1:10-1:250
  • Western Blot 1:1000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections See Simple Western Antibody Database for Simple Western validation: Tested in Jurkat [0.4 mg/mL], separated by Size, antibody dilution of 1:10, 1:50, 1:250, apparent MW was 90-92 kDa

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with , BSA Free
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: >95%

Alternate Names for BRD9 Antibody (BLR153J)

• BRD9
• bromodomain containing 9
• bromodomain-containing protein 9
• DKFZp434D0711
• DKFZp686L0539
• FLJ13441
• FLJ43530
• LAVS3040
• PRO9856
• Rhabdomyosarcoma antigen MU-RMS-40.8
• sarcoma antigen NY-SAR-29

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BRD9