![Western Blot: BRCC3 Antibody (3B1) [NBP1-30429] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat ant imouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: 293T cell lysate Lane 4.: MCF7 cell lysate Lane 5.: NIH3T3 cell lysate.](http://images.novusbio.com/fullsize/BRCC3-Antibody-3B1-Western-Blot-NBP1-30429-img0002.jpg "Western Blot: BRCC3 Antibody (3B1) [NBP1-30429] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat ant imouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: 293T cell lysate Lane 4.: MCF7 cell lysate Lane 5.: NIH3T3 cell lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 3B1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Mouse BRCC3 Antibody (3B1) - BSA Free (NBP1-30429) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Recombinant BRCC3 (1-316aa) purified from E. coli |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BRCC3 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 10% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: BRCC3 Antibody (3B1) [NBP1-30429] - CC/IF analysis of BRCC36 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BRCC36 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/BRCC3-Antibody-3B1-Immunocytochemistry-Immunofluorescence-NBP1-30429-img0006.jpg "Immunocytochemistry/Immunofluorescence: BRCC3 Antibody (3B1) [NBP1-30429] - CC/IF analysis of BRCC36 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BRCC36 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")