BRCC3 Antibody (3B1)

Images

 
Western Blot: BRCC3 Antibody (3B1) [NBP1-30429] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat ant ...read more
Immunocytochemistry/ Immunofluorescence: BRCC3 Antibody (3B1) [NBP1-30429] - CC/IF analysis of BRCC36 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BRCC36 antibody (1:100) ...read more
Flow Cytometry: BRCC3 Antibody (3B1) [NBP1-30429] - Flow cytometry analysis of BRCC36 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
3B1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

BRCC3 Antibody (3B1) Summary

Immunogen
Recombinant BRCC36 (1-316aa) purified from E. coli
Specificity
This antibody is specific for BRCC36 mAb, clone 3B1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BRCC36 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BRCC3
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000-1:2000.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for BRCC3 Antibody (3B1)

  • BRCA1/BRCA2-containing complex subunit 3
  • BRCA1/BRCA2-containing complex subunit 36
  • BRCA1/BRCA2-containing complex, subunit 3
  • BRCA1-A complex subunit BRCC36
  • BRCC36C6.1ACXorf53
  • BRISC complex subunit BRCC36
  • c6.1A
  • chromosome X open reading frame 53
  • EC 3.1.2.15
  • EC 3.4.19.-
  • lys-63-specific deubiquitinase BRCC36

Background

BRCC36 (BRCA1/BRCA2-containing complex, subunit 36) is a holoenzyme complex containing BRCA1, BRCA2. BRCC36 is an ubiquitin E3 ligase complex that has a role in DNA repair and enhances cellular survival following DNA damage. This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BRCC3
Entrez
Uniprot