BRCA1 Antibody (KEN) [DyLight 405]


There are currently no images for BRCA1 Antibody (NB100-599V).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Please see the vial label for concentration. If unlisted please contact technical services.

BRCA1 Antibody (KEN) [DyLight 405] Summary

Human BRCA1 corresponding to residues 1314-1864 [UniProt# P38398]
The epitope recognized is found between amino acids 1314-1600.
IgG3 Kappa
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C in the dark.
DyLight 405
DyLight 405 (A=400, E=420)
50mM Sodium Borate
0.05% Sodium Azide
Protein A purified


  • Western Blot 2-4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This BRCA1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In Western blot a band was observed ~220-240 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
240 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes



Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for BRCA1 Antibody (KEN) [DyLight 405]

  • breast and ovarian cancer susceptibility protein 1
  • breast and ovarian cancer sususceptibility protein
  • breast cancer 1, early onset
  • breast cancer type 1 susceptibility protein
  • EC 6.3.2
  • EC 6.3.2.-
  • IRIS
  • PNCA4
  • PSCP
  • subunit 1


BRCA1 (breast cancer early-onset 1) is a tumour-suppressor gene which was originally discovered on the basis of its linkage to early-onset breast or ovarian cancer in women. BRCA1 gene encodes a protein of 1863 amino acids with three distinct regions of protein interaction: RING domain, RAD51-interaction domain and BRCT (BRCA1 C-terminus) domain. The N-terminal RING finger domain binds to several proteins, including formation of heterodimers with BARD1 (BRCA1-associated RING domain protein). Both BRCA1 and BARD1 contain the RING finger domain which is important for tumour-suppression functions. The RAD51-interaction domain is enclosed by exon 11 and is involved in DNA-DSBs repair. It also contains multiple protein-binding sites, in addition to those for RAD51/RAD50 complex. Loss of RAD51 binding increase the risk of cancer as there could be an increment in the amount of damaged DNA. The C-terminus of BRCA1 contains an acidic domain that functions as a transcriptional activation domain and this domain contains a tandem repeat of approximately 95 amino acids called BRCT. This domain is also found in proteins such as BARD1 which is involved in cell-cycle control and DNA repair. BRCA1 has been implicated in diverse cancer-related activities, including roles in cell-cycle progression, DNA repair, DNA damage-responsive cell-cycle checkpoints, transcription regulation, ubiquitination, chromosome remodelling and apoptosis. Defects in BRCA1 are a cause of susceptibility to breast cancer, familial breast-ovarian cancer type 1 (BROVCA1), ovarian cancer, and pancreatic cancer type 4 (PNCA4).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, IHC
Species: Hu
Applications: WB
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Av, Bv, Pm
Applications: WB, B/N, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: WB, Flow
Species: Hu
Applications: WB, ICC
Species: Hu, Mu, Ce, Ch
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt, Bv, Ch, GP, Pm, Rb, Sh
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: WB, Simple Western, ICC
Species: Hu, Mu, Rt, Pm
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB, IP, PLA
Species: Hu, Mu, Ha, Ma, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IP
Species: Hu
Applications: WB, ICC/IF
Species: Hu, Mu, Rt, Fi, Gt, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu
Applications: WB, IB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Ca
Applications: IP (-), WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Func, ICC/IF, IP
Species: Hu
Applications: WB, ICC/IF, IP

Publications for BRCA1 Antibody (NB100-599V) (0)

There are no publications for BRCA1 Antibody (NB100-599V).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for BRCA1 Antibody (NB100-599V) (0)

There are no reviews for BRCA1 Antibody (NB100-599V). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BRCA1 Antibody (NB100-599V) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-599APC
Biotin Labeled NB100-599B
DyLight 350 Labeled NB100-599UV
DyLight 405LS Labeled NB100-599V3
DyLight 488 Labeled NB100-599G
DyLight 550 Labeled NB100-599R
DyLight 650 Labeled NB100-599C
DyLight 680 Labeled NB100-599FR
DyLight 755 Labeled NB100-599IR
FITC Labeled NB100-599F
HRP Labeled NB100-599H
PE Labeled NB100-599PE
PerCP Labeled NB100-599PCP

Additional BRCA1 Antibody (KEN) [DyLight 405] Products

Related Products by Gene

Bioinformatics Tool for BRCA1 Antibody (NB100-599V)

Discover related pathways, diseases and genes to BRCA1 Antibody (NB100-599V). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BRCA1 Antibody (NB100-599V)

Discover more about diseases related to BRCA1 Antibody (NB100-599V).

Pathways for BRCA1 Antibody (NB100-599V)

View related products by pathway.

PTMs for BRCA1 Antibody (NB100-599V)

Learn more about PTMs related to BRCA1 Antibody (NB100-599V).

Research Areas for BRCA1 Antibody (NB100-599V)

Find related products by research area.

Blogs on BRCA1. Showing 1-10 of 18 blog posts - Show all blog posts.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

FANCD2 (Fanconi anemia subunit D2 protein)
Fanconi anemia (FANC) is a rare, autosomal-recessive genetic disorder that is a heterogeneous cancer susceptibility condition that manifests with a wide range of symptoms such as congenital malformations, deteriorating bone marrow failure, DNA-dama...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

FANCD2: A big component of the DNA repair crew
The genetic disorder known as Fanconi anemia (FANC) is a heterogeneous, autosomal-recessive cancer susceptibility condition characterized by a wide array of symptoms. These include congenital malformations, progressive bone marrow failure, DNA-damage...  Read full blog post.

BRCA1 - A Critical Tumor Suppressor Gene in Women
Breast cancer 1, early onset (BRCA1) is a well-known tumor suppressor gene that was originally discovered due to its link with early-onset breast and ovarian cancer in women. The BRCA1 protein contains the following domains: RING finger, RAD51-interac...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

Breast Cancer Infographic
Breast cancer is caused by malignant cells developing in breast tissue. It is the most commonly diagnosed cancer in women, but advancements in treatment options have seen the death rate decline since the 1990s. Common warning signs of breast cancer in...  Read full blog post.

COBRA1: A Key Player in Transcriptional Pausing
Co-factor of BRCA1, also known as COBRA1, was first identified as a protein that binds to the tumour suppressor protein encoded by the breast cancer susceptibility gene BRCA1 (1). It was subsequently found to be identical to subunit B of the Negative...  Read full blog post.

RAD51: The cell's 'Mr. Fix-it'
RAD51 is a recombinase protein encoded by RAD51 gene in humans. Human RAD51 family members are highly similar to bacterial RecA and yeast Rad51, both biochemically and structurally. It is a 339-amino acid protein that plays an important role in homolo...  Read full blog post.

Showing 1-10 of 18 blog posts - Show all blog posts.

Contact Information

Product PDFs


Gene Symbol BRCA1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB100-599V below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

BRCA1 Recombinant Protein
BRCA1 Antibody Pack
BRCA1 Antibody Pack
BRCA1 Antibody Pack
53BP1 Antibody
53BP1 Antibody
53BP1 Antibody